Product nameAnti-DDX19B antibody
See all DDX19B primary antibodies
DescriptionRabbit polyclonal to DDX19B
SpecificityThe immunogen sequence is highly conserved with DDX19A (GeneID 55308), but crossreactivity with this homolog has not been assessed.
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
Synthetic peptide corresponding to a region between residues 400 and 450 of human DDX19B. (NP_009173.1)
- HeLa, 293T and NIH3T3 whole cell lystes.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).|
|IP||Use at 2 µg/mg of lysate.|
FunctionATP-dependent RNA helicase involved in mRNA export from the nucleus.
Sequence similaritiesBelongs to the DEAD box helicase family. DDX19/DBP5 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Cellular localizationCytoplasm. Nucleus > nuclear pore complex. Nucleus membrane. Nuclear pore complex cytoplasmic fibrils.
- Information by UniProt
- ATP-dependent RNA helicase DDX19B antibody
- DBP5 antibody
- Dbp5, yeast, homolog of antibody
All lanes : Anti-DDX19B antibody (ab70306) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 53 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
ab70306 has not yet been referenced specifically in any publications.