Key features and details
- Rabbit polyclonal to DDX21
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-DDX21 antibody
See all DDX21 primary antibodies
DescriptionRabbit polyclonal to DDX21
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide corresponding to a region between residues 725 and the C-terminal (residue 783) of Human DDX21 (NP_004719.2)
- Human larynx squamous cell carcinoma. Mouse squamous cell carcinoma.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab84394 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionCan unwind double-stranded RNA (helicase) and can fold or introduce a secondary structure to a single-stranded RNA (foldase). Functions as cofactor for JUN-activated transcription. Involved in rRNA processing.
Sequence similaritiesBelongs to the DEAD box helicase family. DDX21/DDX50 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
DomainThe two enzymatic activities reside in two separate domains, the helicase in the N-terminus and the foldase in the C-terminus.
The 3 X 5 AA repeats seem to be critical for the RNA folding activity.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- Ddx21 antibody
- DDX21_HUMAN antibody
- DEAD (Asp-Glu-Ala-Asp) box helicase 21 antibody
Detection of Human DDX21 by Immunohistochemistry.
Sample: FFPE section of Human larynx squamous cell carcinoma.Antibody: ab84394 used at a dilution of 1/250.
Detection of Mouse DDX21 by Immunohistochemistry.
Sample: FFPE section of Mouse squamous cell carcinoma.Antibody: ab84394 used at a dilution of 1/250.
ab84394 has been referenced in 1 publication.
- Lund RR et al. NADH-Cytochrome b5 Reductase 3 Promotes Colonization and Metastasis Formation and Is a Prognostic Marker of Disease-Free and Overall Survival in Estrogen Receptor-Negative Breast Cancer. Mol Cell Proteomics 14:2988-99 (2015). IHC-P ; Human . PubMed: 26351264