Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14495] to DDX21
- Suitable for: Flow Cyt, ICC/IF, IHC-P, WB
- Reacts with: Human
Product nameAnti-DDX21 antibody [EPR14495]
See all DDX21 primary antibodies
DescriptionRabbit monoclonal [EPR14495] to DDX21
Tested applicationsSuitable for: Flow Cyt, ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human DDX21 aa 200-300. The exact sequence is proprietary.
Database link: Q9NR30
- WB: HeLa, and Jurkat lysates. IHC-P: human breast carcinoma tissue. ICC/IF: HeLa cells and HepG2 cells. Flow Cyt: HepG2 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab182156 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
For unpurified use at 1/50 - 1/100
|WB||1/1000 - 1/10000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).|
FunctionCan unwind double-stranded RNA (helicase) and can fold or introduce a secondary structure to a single-stranded RNA (foldase). Functions as cofactor for JUN-activated transcription. Involved in rRNA processing.
Sequence similaritiesBelongs to the DEAD box helicase family. DDX21/DDX50 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
DomainThe two enzymatic activities reside in two separate domains, the helicase in the N-terminus and the foldase in the C-terminus.
The 3 X 5 AA repeats seem to be critical for the RNA folding activity.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- Ddx21 antibody
- DDX21_HUMAN antibody
- DEAD (Asp-Glu-Ala-Asp) box helicase 21 antibody
Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling DDX21 with Purified ab182156 at 1:200 dilution (2.88 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling DDX21 with Purified ab182156 at 1:200 dilution (2.89 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling DDX21 with Purified ab182156 at 1:60 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
All lanes : Anti-DDX21 antibody [EPR14495] (ab182156) at 1/10000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Jurkat (Human Tcellleukemia Tlymphocyte) whole cell lysates
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 87 kDa
Observed band size: 87 kDa
Immunohistochemical analysis of paraffin-embedded Human infiltrating duct carcinoma of breast tissue, staining DDX21 with ab182156 at 1/100 dilution. Hematoxylin was used as a counterstain.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunofluorescence analysis of HeLa cells, staining DDX21 (red) with ab182156 at 1/100 dilution. An Alexa Fluor®555 goat anti rabbit IgG was used as a secondary antibody. Cells were fixed with -20℃ Acetone. Nuclei were counterstained with DAPI (blue).
Immunofluorescence analysis of HepG2 cells, staining DDX21 (green) with ab182156 at 1/100 dilution. An Alexa Fluor®488 goat anti rabbit IgG was used as a secondary antibody. Cells were fixed in 4% paraformaldehyde. Nuclei were counterstained with DAPI (blue).
ab182156 has not yet been referenced specifically in any publications.