Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14496] to DDX21
- Suitable for: WB
- Reacts with: Human
Product nameAnti-DDX21 antibody [EPR14496]
See all DDX21 primary antibodies
DescriptionRabbit monoclonal [EPR14496] to DDX21
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human DDX21 aa 650-750. The exact sequence is proprietary.
Database link: Q9NR30
- HeLa, HT-1080 and HEK293 cell lysate
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab181870 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).|
FunctionCan unwind double-stranded RNA (helicase) and can fold or introduce a secondary structure to a single-stranded RNA (foldase). Functions as cofactor for JUN-activated transcription. Involved in rRNA processing.
Sequence similaritiesBelongs to the DEAD box helicase family. DDX21/DDX50 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
DomainThe two enzymatic activities reside in two separate domains, the helicase in the N-terminus and the foldase in the C-terminus.
The 3 X 5 AA repeats seem to be critical for the RNA folding activity.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- Ddx21 antibody
- DDX21_HUMAN antibody
- DEAD (Asp-Glu-Ala-Asp) box helicase 21 antibody
All lanes : Anti-DDX21 antibody [EPR14496] (ab181870) at 1/2000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 87 kDa
Anti-DDX21 antibody [EPR14496] (ab181870) at 1/10000 dilution + HT-1080 cell lysate at 10 µg
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 87 kDa
ab181870 has not yet been referenced specifically in any publications.