Recombinant
RabMAb

Anti-DDX21 antibody [EPR14496] (ab181870)

Overview

  • Product name
    Anti-DDX21 antibody [EPR14496]
    See all DDX21 primary antibodies
  • Description
    Rabbit monoclonal [EPR14496] to DDX21
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human DDX21 aa 650-750. The exact sequence is proprietary.
    Database link: Q9NR30

  • Positive control
    • HeLa, HT-1080 and HEK293 cell lysate
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).

Target

  • Function
    Can unwind double-stranded RNA (helicase) and can fold or introduce a secondary structure to a single-stranded RNA (foldase). Functions as cofactor for JUN-activated transcription. Involved in rRNA processing.
  • Sequence similarities
    Belongs to the DEAD box helicase family. DDX21/DDX50 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Domain
    The two enzymatic activities reside in two separate domains, the helicase in the N-terminus and the foldase in the C-terminus.
    The 3 X 5 AA repeats seem to be critical for the RNA folding activity.
  • Cellular localization
    Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ddx21 antibody
    • DDX21_HUMAN antibody
    • DEAD (Asp-Glu-Ala-Asp) box helicase 21 antibody
    • DEAD (Asp-Glu-Ala-Asp) box polypeptide 21 antibody
    • DEAD box protein 21 antibody
    • EC 3.6.1.- antibody
    • Gu alpha antibody
    • Gu protein antibody
    • Gu-alpha antibody
    • GUA antibody
    • GURDB antibody
    • Nucleolar RNA helicase 2 antibody
    • Nucleolar RNA helicase Gu antibody
    • Nucleolar RNA helicase II antibody
    • RH II/Gu antibody
    • RH II/GuA antibody
    • RNA helicase II/Gu alpha antibody
    see all

Images

  • All lanes : Anti-DDX21 antibody [EPR14496] (ab181870) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 87 kDa

  • Anti-DDX21 antibody [EPR14496] (ab181870) at 1/10000 dilution + HT-1080 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 87 kDa

References

ab181870 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181870.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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