Overview

  • Product name

  • Description

    Rabbit polyclonal to DDX23
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human DDX23. The exact sequence is proprietary.
    Database link: Q9BUQ8

  • Positive control

    • WB: HeLa, NIH-3T3, JC whole cell lysates. IHC-P: HBL435 xenograft. ICC/IF: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 96 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Involved in pre-mRNA splicing and its phosphorylated form (by SRPK2) is required for spliceosomal B complex formation.
  • Sequence similarities

    Belongs to the DEAD box helicase family. DDX23/PRP28 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Post-translational
    modifications

    In vitro phosphorylated by CLK1 and U1 snRNP-associated protein kinase. Phosphorylated by SRPK2 and this phosphorylation is required for its association with the tri-snRNP (U4/U6-U5 tri-small nuclear ribonucleoproteins) and subsequent spliceosomal B complex formation.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 100 kDa U5 snRNP-specific protein antibody
    • DDX23 antibody
    • DDX23_HUMAN antibody
    • DEAD box protein 23 antibody
    • EC 3.6.1.- antibody
    • Probable ATP-dependent RNA helicase DDX23 antibody
    • PRP28 homolog antibody
    • U5-100kD antibody
    see all

Images

  • All lanes : Anti-DDX23 antibody (ab228999) at 1/1000 dilution

    Lane 1 : NIH-3T3 (mouse embryo fibroblast cell line) whole cell lysate.
    Lane 2 : JC (Mouse mammary gland cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 96 kDa



    7.5% SDS-PAGE gel.

  • Paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma cell line) cells stained for DDX23 protein using ab228999 at 1/500 dilution in ICC/IF.

    Alpha-tubulin filaments (red).

     

  • Anti-DDX23 antibody (ab228999) at 1/1000 dilution + HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 96 kDa



    7.5% SDS-PAGE gel.

  • Paraffin-embedded HBL435 xenograft tissue stained for DDX23 protein using ab228999 at 1/500 dilution in immunohistochemical analysis.

References

ab228999 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228999.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up