Product nameAnti-DDX24 antibody
See all DDX24 primary antibodies
DescriptionRabbit polyclonal to DDX24
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residue 325 and 375 of Human DDX24 (NP_065147.1)
- HeLa, 293T and NIH3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70463 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionATP-dependent RNA helicase.
Tissue specificityUbiquitous. Most abundant in heart and brain, but with lowest levels in thymus and small intestine.
Sequence similaritiesBelongs to the DEAD box helicase family. DDX24/MAK5 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- ATP dependent RNA helicase DDX24 antibody
- ATP-dependent RNA helicase ddx24 antibody
- DDX 24 antibody
All lanes : Anti-DDX24 antibody (ab70463) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 97 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Additional bands at: 160 kDa, 230 kDa, 350 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 seconds
ab70463 has not yet been referenced specifically in any publications.