Anti-DDX27 antibody [2251C2a] (ab50696)
![Western blot - Anti-DDX27 antibody [2251C2a] (ab50696)](https://www.abcam.com/ps/products/50/ab50696/Images/ab506962.gif "Western blot - Anti-DDX27 antibody [2251C2a] (ab50696)")
Key features and details
- Mouse monoclonal [2251C2a] to DDX27
- Suitable for: WB, ICC
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-DDX27 antibody [2251C2a]
See all DDX27 primary antibodies -
Description
Mouse monoclonal [2251C2a] to DDX27 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanWB MouseHuman
-
Immunogen
Recombinant human DDX27
-
Positive control
- HeLa cells and HeLa and NIH3T3 cell lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.0225% Potassium chloride, 0.03% Potassium phosphate, 0.1312% Sodium phosphate, 0.812% Sodium chloride, 1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab50696 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22 µm membrane. -
Clonality
Monoclonal -
Clone number
2251C2a -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab50696 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC |
Human
|
| WB |
Mouse
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/50. Predicted molecular weight: 90 kDa.
|
|
| ICC |
1/5.
|
| Notes |
|---|
|
WB
1/50. Predicted molecular weight: 90 kDa. |
|
ICC
1/5. |
Target
-
Function
Probable ATP-dependent RNA helicase. -
Sequence similarities
Belongs to the DEAD box helicase family. DDX27/DRS1 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 55661 Human
- Entrez Gene: 228889 Mouse
- SwissProt: Q96GQ7 Human
- SwissProt: Q921N6 Mouse
- Unigene: 404263 Human
- Unigene: 65234 Human
- Unigene: 295031 Mouse
-
Alternative names
- C86129 antibody
- cb843 antibody
- DDX27 antibody
see all
Images
-
Western blot - Anti-DDX27 antibody [2251C2a] (ab50696)Anti-DDX27 antibody [2251C2a] (ab50696) at 1/50 dilution + HeLa whole cell lysate at 25 µg
Secondary
Mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 90 kDa
Observed band size: 90 kDa -
![Western blot - Anti-DDX27 antibody [2251C2a] (ab50696)](https://www.abcam.com/ps/products/50/ab50696/Images/ab50696wb2.gif)
Western blot - Anti-DDX27 antibody [2251C2a] (ab50696)Anti-DDX27 antibody [2251C2a] (ab50696) at 1/50 dilution + NIH3T3 whole cell lyaste at 25 µg
Secondary
Mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 90 kDa
Observed band size: 90 kDa -
![Immunocytochemistry - Anti-DDX27 antibody [2251C2a] (ab50696)](https://www.abcam.com/ps/products/50/ab50696/Images/ab50696icc.gif)
Immunocytochemistry - Anti-DDX27 antibody [2251C2a] (ab50696)ab50696 at 1/5 dilution staining human DDX27 in HeLa cells by Immunocytochemistry. A 1/200 dilution of AlexaFluor488 Goat Anti-mouse IgG was used as secondary.
Protocols
Datasheets and documents
References (0)
ab50696 has not yet been referenced specifically in any publications.
