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    ddx27-antibody-2251c2a-ab50696.pdf

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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing Other
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Anti-DDX27 antibody [2251C2a] (ab50696)

  • Datasheet
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Western blot - Anti-DDX27 antibody [2251C2a] (ab50696)
  • Western blot - Anti-DDX27 antibody [2251C2a] (ab50696)
  • Immunocytochemistry - Anti-DDX27 antibody [2251C2a] (ab50696)

Key features and details

  • Mouse monoclonal [2251C2a] to DDX27
  • Suitable for: WB, ICC
  • Reacts with: Mouse, Human
  • Isotype: IgG1

You may also be interested in

Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

View more associated products

Overview

  • Product name

    Anti-DDX27 antibody [2251C2a]
    See all DDX27 primary antibodies
  • Description

    Mouse monoclonal [2251C2a] to DDX27
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    ICC
    Human
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Recombinant human DDX27

  • Positive control

    • HeLa cells and HeLa and NIH3T3 cell lysates

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.0225% Potassium chloride, 0.03% Potassium phosphate, 0.1312% Sodium phosphate, 0.812% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    ab50696 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22 µm membrane.
  • Clonality

    Monoclonal
  • Clone number

    2251C2a
  • Isotype

    IgG1
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
    • NIH/3T3 nuclear extract lysate (ab14874)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab50696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC
Human
WB
Mouse
Human
Application Abreviews Notes
WB
1/50. Predicted molecular weight: 90 kDa.
ICC
1/5.
Notes
WB
1/50. Predicted molecular weight: 90 kDa.
ICC
1/5.

Target

  • Function

    Probable ATP-dependent RNA helicase.
  • Sequence similarities

    Belongs to the DEAD box helicase family. DDX27/DRS1 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q96GQ7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 55661 Human
    • Entrez Gene: 228889 Mouse
    • SwissProt: Q96GQ7 Human
    • SwissProt: Q921N6 Mouse
    • Unigene: 404263 Human
    • Unigene: 65234 Human
    • Unigene: 295031 Mouse
    • Alternative names

      • C86129 antibody
      • cb843 antibody
      • DDX27 antibody
      • DDX27_HUMAN antibody
      • DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 antibody
      • DEAD box protein 27 antibody
      • Deficiency of ribosomal subunits protein 1 homolog antibody
      • dJ686N3.1 antibody
      • DKFZp667N057 antibody
      • DRS1 antibody
      • FLJ12917 antibody
      • FLJ20596 antibody
      • FLJ22238 antibody
      • HSPC259 antibody
      • MGC1018 antibody
      • MGC163147 antibody
      • PP3241 antibody
      • Probable ATP-dependent RNA helicase DDX27 antibody
      • RHLP antibody
      • RNA helicase like protein antibody
      • Rrp3p antibody
      see all

    Images

    • Western blot - Anti-DDX27 antibody [2251C2a] (ab50696)
      Western blot - Anti-DDX27 antibody [2251C2a] (ab50696)
      Anti-DDX27 antibody [2251C2a] (ab50696) at 1/50 dilution + HeLa whole cell lysate at 25 µg

      Secondary
      Mouse IgG at 1/2500 dilution

      Developed using the ECL technique.

      Predicted band size: 90 kDa
      Observed band size: 90 kDa

    • Western blot - Anti-DDX27 antibody [2251C2a] (ab50696)
      Western blot - Anti-DDX27 antibody [2251C2a] (ab50696)
      Anti-DDX27 antibody [2251C2a] (ab50696) at 1/50 dilution + NIH3T3 whole cell lyaste at 25 µg

      Secondary
      Mouse IgG at 1/2500 dilution

      Developed using the ECL technique.

      Predicted band size: 90 kDa
      Observed band size: 90 kDa

    • Immunocytochemistry - Anti-DDX27 antibody [2251C2a] (ab50696)
      Immunocytochemistry - Anti-DDX27 antibody [2251C2a] (ab50696)
      ab50696 at 1/5 dilution staining human DDX27 in HeLa cells by Immunocytochemistry. A 1/200 dilution of AlexaFluor488 Goat Anti-mouse IgG was used as secondary.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab50696? Please let us know so that we can cite the reference in this datasheet.

    ab50696 has not yet been referenced specifically in any publications.

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