- Datasheet
- Specific References
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Overview
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Product nameAnti-DDX27 antibody [2251C2a]
See all DDX27 primary antibodies -
DescriptionMouse monoclonal [2251C2a] to DDX27
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Host speciesMouse
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Tested applicationsSuitable for: WB, Dot blot, ICCmore details
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Species reactivityReacts with: Mouse, Human
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Immunogen
Recombinant human DDX27
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Positive control
- HeLa cells and HeLa and NIH3T3 cell lysates
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.0225% Potassium chloride, 0.03% Potassium phosphate, 0.1312% Sodium phosphate, 0.812% Sodium chloride, 1% BSA -
Concentration information loading... -
PurityProtein G purified
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Purification notesab50696 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22 µm membrane.
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ClonalityMonoclonal
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Clone number2251C2a
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab50696 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/50. Predicted molecular weight: 90 kDa. | |
| Dot blot | Use at an assay dependent dilution. | |
| ICC | 1/5. |
Target
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FunctionProbable ATP-dependent RNA helicase.
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Sequence similaritiesBelongs to the DEAD box helicase family. DDX27/DRS1 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 55661 Human
- Entrez Gene: 228889 Mouse
- SwissProt: Q96GQ7 Human
- SwissProt: Q921N6 Mouse
- Unigene: 404263 Human
- Unigene: 65234 Human
- Unigene: 295031 Mouse
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Alternative names
- C86129 antibody
- cb843 antibody
- DDX27 antibody
see all
Images
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![Western blot - Anti-DDX27 antibody [2251C2a] (ab50696)](https://www.abcam.com/ps/products/50/ab50696/Images/ab506962.gif)
Western blot - Anti-DDX27 antibody [2251C2a] (ab50696)Anti-DDX27 antibody [2251C2a] (ab50696) at 1/50 dilution + HeLa whole cell lysate at 25 µg
Secondary
Mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 90 kDa
Observed band size: 90 kDa -
![Western blot - Anti-DDX27 antibody [2251C2a] (ab50696)](https://www.abcam.com/ps/products/50/ab50696/Images/ab50696wb2.gif)
Western blot - Anti-DDX27 antibody [2251C2a] (ab50696)Anti-DDX27 antibody [2251C2a] (ab50696) at 1/50 dilution + NIH3T3 whole cell lyaste at 25 µg
Secondary
Mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 90 kDa
Observed band size: 90 kDa -
![Immunocytochemistry - Anti-DDX27 antibody [2251C2a] (ab50696)](https://www.abcam.com/ps/products/50/ab50696/Images/ab50696icc.gif)
Immunocytochemistry - Anti-DDX27 antibody [2251C2a] (ab50696)ab50696 at 1/5 dilution staining human DDX27 in HeLa cells by Immunocytochemistry. A 1/200 dilution of AlexaFluor488 Goat Anti-mouse IgG was used as secondary.
References
ab50696 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"