Recombinant
RabMAb

Recombinant Anti-DDX27 antibody [EPR13147] (ab177950)

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Overview

  • Product name

    Anti-DDX27 antibody [EPR13147]
    See all DDX27 primary antibodies

  • Description

    Rabbit monoclonal [EPR13147] to DDX27

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DDX27 aa 750 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q96GQ7

  • Positive control

    • HeLa, HepG2 and Jurkat whole cell lysate (ab7899); HeLa cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR13147

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab177950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 89 kDa.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.

    • Target

    • Function

      Probable ATP-dependent RNA helicase.

    • Sequence similarities

      Belongs to the DEAD box helicase family. DDX27/DRS1 subfamily.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • C86129 antibody
      • cb843 antibody
      • DDX27 antibody
      • DDX27_HUMAN antibody
      • DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 antibody
      • DEAD box protein 27 antibody
      • Deficiency of ribosomal subunits protein 1 homolog antibody
      • dJ686N3.1 antibody
      • DKFZp667N057 antibody
      • DRS1 antibody
      • FLJ12917 antibody
      • FLJ20596 antibody
      • FLJ22238 antibody
      • HSPC259 antibody
      • MGC1018 antibody
      • MGC163147 antibody
      • PP3241 antibody
      • Probable ATP-dependent RNA helicase DDX27 antibody
      • RHLP antibody
      • RNA helicase like protein antibody
      • Rrp3p antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-DDX27 antibody [EPR13147] (ab177950)
      Immunocytochemistry/ Immunofluorescence - Anti-DDX27 antibody [EPR13147] (ab177950)

      Immunofluorescent analysis of HeLa cells labeling DDX27 with ab177950 at 1/50 dilution.

    • Western blot - Anti-DDX27 antibody [EPR13147] (ab177950)
      Western blot - Anti-DDX27 antibody [EPR13147] (ab177950)
      All lanes : Anti-DDX27 antibody [EPR13147] (ab177950) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 89 kDa

    References

    ab177950 has not yet been referenced specifically in any publications.

    Publishing research using ab177950? Please let us know so that we can cite the reference in this datasheet.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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