
Recombinant Anti-DDX27 antibody [EPR13147] (ab177950)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-DDX27 antibody [EPR13147]
See all DDX27 primary antibodies -
Description
Rabbit monoclonal [EPR13147] to DDX27 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DDX27 aa 750 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q96GQ7 -
Positive control
- HeLa, HepG2 and Jurkat whole cell lysate (ab7899); HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13147 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab177950 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/1000 - 1/5000. Predicted molecular weight: 89 kDa. | |
ICC/IF | 1/50 - 1/100. |
Target
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Function
Probable ATP-dependent RNA helicase. -
Sequence similarities
Belongs to the DEAD box helicase family. DDX27/DRS1 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 55661 Human
- Entrez Gene: 228889 Mouse
- Entrez Gene: 362274 Rat
- SwissProt: Q96GQ7 Human
- SwissProt: Q921N6 Mouse
- Unigene: 404263 Human
- Unigene: 65234 Human
- Unigene: 295031 Mouse
see all -
Alternative names
- C86129 antibody
- cb843 antibody
- DDX27 antibody
see all
Images
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Immunofluorescent analysis of HeLa cells labeling DDX27 with ab177950 at 1/50 dilution.
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All lanes : Anti-DDX27 antibody [EPR13147] (ab177950) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 89 kDa
Datasheets and documents
References
ab177950 has not yet been referenced specifically in any publications.