Overview

  • Product name

  • Description

    Rabbit polyclonal to DDX28
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 126-341 of Human DDX28 (Q9NUL7).

  • Positive control

    • NT2D1 and IMR32 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab154667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 60 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    May be involved in RNA processing or transport. Has RNA and Mg(2+)-dependent ATPase activity.
  • Tissue specificity

    Expressed in all tissues tested, including brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, leukocytes, colon, small intestine, ovary and prostate.
  • Sequence similarities

    Belongs to the DEAD box helicase family.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localization

    Nucleus. Mitochondrion. Transported between these two compartments. Nuclear localization depends on active RNA polymerase II transcription.
  • Information by UniProt
  • Database links

  • Alternative names

    • DDX 28 antibody
    • DDX28 antibody
    • DDX28_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 28 antibody
    • DEAD box polypeptide 28 antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 28 antibody
    • FLJ11282 antibody
    • MDDX 28 antibody
    • MDDX28 antibody
    • Mitochondrial DEAD box protein 28 antibody
    • Probable ATP-dependent RNA helicase DDX28 antibody
    see all

Images

  • Anti-DDX28 antibody (ab154667) at 1/1000 dilution + IMR32 whole cell lysate at 30 µg

    Predicted band size: 60 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded Cal27 xenograft tissue labelling DDX28 with ab154667 at a 1/500 dilution.

References

ab154667 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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