Anti-DDX28 antibody (ab70821)

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Overview

  • Product name

    Anti-DDX28 antibody
    See all DDX28 primary antibodies

  • Description

    Rabbit polyclonal to DDX28

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla

  • Immunogen

    Synthetic peptide corresponding to a region between residue 500 and the C-terminus (residue 540) of human DDX28 (NP_060850.1).

  • Positive control

    • HeLa and 293T whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab70821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
WB
  • Application notes
    IP: Use at 2-5µg/mg of lysate.
    WB: 1/1000 - 1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function

      May be involved in RNA processing or transport. Has RNA and Mg(2+)-dependent ATPase activity.

    • Tissue specificity

      Expressed in all tissues tested, including brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, leukocytes, colon, small intestine, ovary and prostate.

    • Sequence similarities

      Belongs to the DEAD box helicase family.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.

    • Cellular localization

      Nucleus. Mitochondrion. Transported between these two compartments. Nuclear localization depends on active RNA polymerase II transcription.
    • Information by UniProt

    • Database links

      • Alternative names

        • DDX 28 antibody
        • DDX28 antibody
        • DDX28_HUMAN antibody
        • DEAD (Asp Glu Ala Asp) box polypeptide 28 antibody
        • DEAD box polypeptide 28 antibody
        • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 28 antibody
        • FLJ11282 antibody
        • MDDX 28 antibody
        • MDDX28 antibody
        • Mitochondrial DEAD box protein 28 antibody
        • Probable ATP-dependent RNA helicase DDX28 antibody
        see all

      Images

      • Western blot - Anti-DDX28 antibody (ab70821)
        Western blot - Anti-DDX28 antibody (ab70821)
        All lanes : Anti-DDX28 antibody (ab70821) at 0.4 µg/ml

        Lane 1 : HeLa whole cell lysate at 50 µg
        Lane 2 : HeLa whole cell lysate at 15 µg
        Lane 3 : HeLa whole cell lysate at 5 µg
        Lane 4 : 293T whole cell lysate at 50 µg

        Predicted band size: 60 kDa
        Observed band size: 60 kDa
        Additional bands at: 28 kDa. We are unsure as to the identity of these extra bands.

      • Immunoprecipitation - Anti-DDX28 antibody (ab70821)
        Immunoprecipitation - Anti-DDX28 antibody (ab70821)
        Detection of Human DDX28 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70821 at 3 µg/mg lysate (Lane 1). Lane 2 represents rabbit IgG IP control. Subsequent Western blot detection of DDX28 was performed using ab70821 at 1 µg/ml.

      References

      ab70821 has not yet been referenced specifically in any publications.

      Publishing research using ab70821? Please let us know so that we can cite the reference in this datasheet.

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