Anti-DDX28 antibody (ab70821)

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Western blot - Anti-DDX28 antibody (ab70821)
  • Immunoprecipitation - Anti-DDX28 antibody (ab70821)

Key features and details

  • Rabbit polyclonal to DDX28
  • Suitable for: IP, WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-DDX28 antibody
    See all DDX28 primary antibodies

  • Description

    Rabbit polyclonal to DDX28

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to a region between residue 500 and the C-terminus (residue 540) of human DDX28 (NP_060850.1).

  • Positive control

    • HeLa and 293T whole cell lysates.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab70821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IP
Human
WB
Human
All applications
Chimpanzee
Gorilla
Application Abreviews Notes
IP
Use at 2 µg/mg of lysate.
WB
1/1000 - 1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
Notes
IP
Use at 2 µg/mg of lysate.
WB
1/1000 - 1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).

Target

  • Function

    May be involved in RNA processing or transport. Has RNA and Mg(2+)-dependent ATPase activity.

  • Tissue specificity

    Expressed in all tissues tested, including brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, leukocytes, colon, small intestine, ovary and prostate.

  • Sequence similarities

    Belongs to the DEAD box helicase family.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.

  • Cellular localization

    Nucleus. Mitochondrion. Transported between these two compartments. Nuclear localization depends on active RNA polymerase II transcription.
  • Information by UniProt

  • Database links

    • Alternative names

      • DDX 28 antibody
      • DDX28 antibody
      • DDX28_HUMAN antibody
      • DEAD (Asp Glu Ala Asp) box polypeptide 28 antibody
      • DEAD box polypeptide 28 antibody
      • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 28 antibody
      • FLJ11282 antibody
      • MDDX 28 antibody
      • MDDX28 antibody
      • Mitochondrial DEAD box protein 28 antibody
      • Probable ATP-dependent RNA helicase DDX28 antibody
      see all

    Images

    • Western blot - Anti-DDX28 antibody (ab70821)
      Western blot - Anti-DDX28 antibody (ab70821)
      All lanes : Anti-DDX28 antibody (ab70821) at 0.4 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Predicted band size: 60 kDa
      Observed band size: 60 kDa
      Additional bands at: 28 kDa. We are unsure as to the identity of these extra bands.

    • Immunoprecipitation - Anti-DDX28 antibody (ab70821)
      Immunoprecipitation - Anti-DDX28 antibody (ab70821)
      Detection of Human DDX28 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70821 at 3 µg/mg lysate (Lane 1). Lane 2 represents rabbit IgG IP control. Subsequent Western blot detection of DDX28 was performed using ab70821 at 1 µg/ml.

    References (1)

    Publishing research using ab70821? Please let us know so that we can cite the reference in this datasheet.

    ab70821 has been referenced in 1 publication.

    • Reyes A  et al. RNase H1 Regulates Mitochondrial Transcription and Translation via the Degradation of 7S RNA. Front Genet 10:1393 (2019). PubMed: 32082360

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