Overview

  • Product name

    Anti-DDX3 antibody [6G8-F4-E3]
    See all DDX3 primary antibodies
  • Description

    Mouse monoclonal [6G8-F4-E3] to DDX3
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Monkey
  • Immunogen

    Recombinant fragment corresponding to Human DDX3. (Purified fragments expressed in E.coli; NM_001356).
    Database link: O00571

  • Positive control

    • Jurkat, K562,COS7, C6, 3T3 and HeLa whole cell lysate (ab150035); HeLa cells.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 17.08.2018.  Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab196032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 73 kDa.
IP Use at an assay dependent concentration.
ICC/IF 1/200.

Target

  • Function

    ATP-dependent RNA helicase. Acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. Also involved in HIV-1 replication. Interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location.
  • Sequence similarities

    Belongs to the DEAD box helicase family. DDX3/DED1 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localization

    Nucleus speckle. Cytoplasm. Located predominantly in nuclear speckles and, at low levels, throughout the cytoplasm. Located to the outer side of nuclear pore complexes (NPC). Shuttles between the nucleus and the cytoplasm in a XPO1-dependent manner.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATP dependent RNA helicase DDX3X antibody
    • ATP-dependent RNA helicase DDX3X antibody
    • CAP Rf antibody
    • DBX antibody
    • DDX14 antibody
    • DDX3X antibody
    • DDX3X_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 3 X linked antibody
    • DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked antibody
    • DEAD box antibody
    • DEAD box protein 3 antibody
    • DEAD box protein 3 X-chromosomal antibody
    • DEAD box X isoform antibody
    • DEAD box, X isoform antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 3 antibody
    • DEAD/H box 3 antibody
    • DEAD/H box 3, X-linked antibody
    • Helicase like protein 2 antibody
    • Helicase-like protein 2 antibody
    • HLP2 antibody
    • X isoform antibody
    • X-chromosomal antibody
    see all

Images

  • All lanes : Anti-DDX3 antibody [6G8-F4-E3] (ab196032) at 1/1000 dilution

    Lane 1 : COS7 Cell lysates
    Lane 2 : Jurkat Cell lysates
    Lane 3 : C6 Cell lysates
    Lane 4 : 3T3 Cell lysates
    Lane 5 : HeLa Cell lysates

    Predicted band size: 73 kDa

  • All lanes : Anti-DDX3 antibody [6G8-F4-E3] (ab196032) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : COS7 cell lysate
    Lane 4 : C6 cell lysate
    Lane 5 : 3T3 cell lysate
    Lane 6 : HeLa cell lysate

    Predicted band size: 73 kDa



    This image was produced with antibody produced in ascites. 

  • Immunofluorescent analysis of 4% parafomaldehyde-fixed HeLa cells labeling DDX3 with ab196032 at 1/200 dilution. This image was produced with antibody produced in ascites. 

  • Detection of DDX3 in immunoprecipitates of HeLa cell lysate immunoprecipitated using ab196032 (Lane 2). Lane 1 represents control IgG IP. This image was produced with antibody produced in ascites. 

References

This product has been referenced in:

  • Su YS  et al. Stimulation of the Internal Ribosome Entry Site (IRES)-Dependent Translation of Enterovirus 71 by DDX3X RNA Helicase and Viral 2A and 3C Proteases. Front Microbiol 9:1324 (2018). Read more (PubMed: 29971060) »
  • Cannizzaro E  et al. DDX3X RNA helicase affects breast cancer cell cycle progression by regulating expression of KLF4. FEBS Lett 592:2308-2322 (2018). Read more (PubMed: 29782654) »
See all 2 Publications for this product

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