Key features and details
- Rabbit polyclonal to DDX3
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-DDX3 antibody
See all DDX3 primary antibodies
DescriptionRabbit polyclonal to DDX3
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Purified full-length Human DDX3X with a proprietary tag.
- HeLa whole cell extracts; HeLa cells.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6
Preservative: 0.05% Sodium azide
Constituent: 99% Whole serum
Concentration information loading...
Our Abpromise guarantee covers the use of ab128206 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 73 kDa.|
FunctionATP-dependent RNA helicase. Acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. Also involved in HIV-1 replication. Interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location.
Sequence similaritiesBelongs to the DEAD box helicase family. DDX3/DED1 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Cellular localizationNucleus speckle. Cytoplasm. Located predominantly in nuclear speckles and, at low levels, throughout the cytoplasm. Located to the outer side of nuclear pore complexes (NPC). Shuttles between the nucleus and the cytoplasm in a XPO1-dependent manner.
- Information by UniProt
- ATP dependent RNA helicase DDX3X antibody
- ATP-dependent RNA helicase DDX3X antibody
- CAP Rf antibody
Anti-DDX3 antibody (ab128206) at 1/1000 dilution + HeLa whole cell extracts at 10 µg
Predicted band size: 73 kDa
ab128206, at 1/500, staining DDX3 in untreated (a) or LMB-treated (b) Paraformaldehyde-fixed HeLa cells by Immunoflouroscence (green).
DDX3X is localized in cytoplasm in the absence of treatment, but once treated with LMB, a nuclear translocation inhibitor, it is localized not only in cytoplasm but also in nuclei.
ab128206 has been referenced in 1 publication.
- Sekiguchi T et al. Human DDX3Y, the Y-encoded isoform of RNA helicase DDX3, rescues a hamster temperature-sensitive ET24 mutant cell line with a DDX3X mutation. Exp Cell Res 300:213-22 (2004). PubMed: 15383328