Overview

  • Product name

  • Description

    Rabbit polyclonal to DDX3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Purified full-length Human DDX3X with a proprietary tag.

  • Positive control

    • HeLa whole cell extracts; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab128206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 73 kDa.
ICC/IF 1/500.

Target

  • Function

    ATP-dependent RNA helicase. Acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. Also involved in HIV-1 replication. Interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location.
  • Sequence similarities

    Belongs to the DEAD box helicase family. DDX3/DED1 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localization

    Nucleus speckle. Cytoplasm. Located predominantly in nuclear speckles and, at low levels, throughout the cytoplasm. Located to the outer side of nuclear pore complexes (NPC). Shuttles between the nucleus and the cytoplasm in a XPO1-dependent manner.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATP dependent RNA helicase DDX3X antibody
    • ATP-dependent RNA helicase DDX3X antibody
    • CAP Rf antibody
    • DBX antibody
    • DDX14 antibody
    • DDX3X antibody
    • DDX3X_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 3 X linked antibody
    • DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked antibody
    • DEAD box antibody
    • DEAD box protein 3 antibody
    • DEAD box protein 3 X-chromosomal antibody
    • DEAD box X isoform antibody
    • DEAD box, X isoform antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 3 antibody
    • DEAD/H box 3 antibody
    • DEAD/H box 3, X-linked antibody
    • Helicase like protein 2 antibody
    • Helicase-like protein 2 antibody
    • HLP2 antibody
    • X isoform antibody
    • X-chromosomal antibody
    see all

Images

  • Anti-DDX3 antibody (ab128206) at 1/1000 dilution + HeLa whole cell extracts at 10 µg

    Predicted band size: 73 kDa

  • ab128206, at 1/500, staining DDX3 in untreated (a) or LMB-treated (b) Paraformaldehyde-fixed HeLa cells by Immunoflouroscence (green).
    DDX3X is localized in cytoplasm in the absence of treatment, but once treated with LMB, a nuclear translocation inhibitor, it is localized not only in cytoplasm but also in nuclei.

References

This product has been referenced in:

  • Sekiguchi T  et al. Human DDX3Y, the Y-encoded isoform of RNA helicase DDX3, rescues a hamster temperature-sensitive ET24 mutant cell line with a DDX3X mutation. Exp Cell Res 300:213-22 (2004). Read more (PubMed: 15383328) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
18 µg
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Pig Cell lysate - whole cell (New born porcine tracheal epithelial cell line)
Specification
New born porcine tracheal epithelial cell line
Treatment
Influenza infection
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C

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Verified customer

Submitted Sep 09 2013

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