Overview

  • Product name
  • Description
    Rabbit polyclonal to DDX3
  • Host species
    Rabbit
  • Specificity
    Detects both DDX3 and DBY
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to a 16 amino acid peptide from near the N terminus of Human DDX3

  • Positive control
    • HepG2 (Human hepatocellular liver carcinoma cell line) whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab37160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    ATP-dependent RNA helicase. Acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. Also involved in HIV-1 replication. Interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location.
  • Sequence similarities
    Belongs to the DEAD box helicase family. DDX3/DED1 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localization
    Nucleus speckle. Cytoplasm. Located predominantly in nuclear speckles and, at low levels, throughout the cytoplasm. Located to the outer side of nuclear pore complexes (NPC). Shuttles between the nucleus and the cytoplasm in a XPO1-dependent manner.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP dependent RNA helicase DDX3X antibody
    • ATP-dependent RNA helicase DDX3X antibody
    • CAP Rf antibody
    • DBX antibody
    • DDX14 antibody
    • DDX3X antibody
    • DDX3X_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 3 X linked antibody
    • DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked antibody
    • DEAD box antibody
    • DEAD box protein 3 antibody
    • DEAD box protein 3 X-chromosomal antibody
    • DEAD box X isoform antibody
    • DEAD box, X isoform antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 3 antibody
    • DEAD/H box 3 antibody
    • DEAD/H box 3, X-linked antibody
    • Helicase like protein 2 antibody
    • Helicase-like protein 2 antibody
    • HLP2 antibody
    • X isoform antibody
    • X-chromosomal antibody
    see all

Images

  • Lane 1 : Anti-DDX3 antibody (ab37160) at 0.5 µg/ml
    Lane 2 : Anti-DDX3 antibody (ab37160) at 1 µg/ml

    All lanes : Human HepG2 cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 73 kDa
    Observed band size: 73 kDa

  • Immunofluorescence of DDX3 in HepG2 cells using ab37160 at 20 ug/ml.

References

This product has been referenced in:
  • Wang J  et al. RNA-binding protein PSPC1 promotes the differentiation-dependent nuclear export of adipocyte RNAs. J Clin Invest 127:987-1004 (2017). Read more (PubMed: 28192372) »
  • King HA  et al. Remodelling of a polypyrimidine tract-binding protein complex during apoptosis activates cellular IRESs. Cell Death Differ N/A:N/A (2013). Read more (PubMed: 24141718) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (Vascular smooth muscle cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Vascular smooth muscle cells
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 21°C

Abcam user community

Verified customer

Submitted Nov 24 2017

Answer

Thank you for your enquiry. I am sorry to hear you are having a problem with ab37160. I would like to suggest the following modifications to your protocol: -Run a positive control of HepG2 (Human hepatocellular liver carcinoma cell line) whole cell lysate (we have some in our catalogue if you need to purchase this, reference ab7900) as it may be that your samples express small amount of the DDX3 protein, which are not easily detected in western blotting. -As you have not specified the type of lysis buffer you use, I would like to make sure you use a strong buffer, such as RIPA buffer, to extract the protein, as it is located predominantly in the nucleus. It may be that a nuclear extraction procedure is needed to concentrate the protein in your samples. I would be happy to provide you details of those protocols if you need those, please do not hesitate to ask for them. -Incubate the membrane with primary antibody overnight, at 4C, to maximise the binding of the primary to the protein. -Use an ecl+ or superSignal detection kit, as these are more sensitive than Ecl and will give you stronger signal. -The antibody has only been tested in reducing conditions, therefore it may be that it only works in those conditions. I would recommend switching to a reducing gel and also making sure the conditions are denaturing too. -I am not sure if you mean you block overnight, or transfer overnight. I would recommend to block only 1 hour at room temperature, and trying both 5% milk and 5% BSA as some antibodies prefer one blocking agent over another. -Please also make sure the secondary antibody works well with other primary antibodies, as it may be loosing activity and not be working well. Please let me know if this helps and do not hesitate to contact me again if you still experience problems with the positive control and the recommended changes. I would be happy to offer you a replacement vial or refund if the antibody was bought in the last 120 days and it still does not perform after those tips.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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