Product nameAnti-DDX3 antibody
See all DDX3 primary antibodies
DescriptionRabbit polyclonal to DDX3
SpecificityDetects endogenous levels of total DDX3 protein
Tested applicationsSuitable for: ICC/IF, ELISA, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic non phosphopeptide derived from human DDX3 around the phosphorylation site of threonine 322 (VATPPG).
- Human brain tissue (IHC) Extracts from HepG2 cells (WB)
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from rabbit antiserum
Our Abpromise guarantee covers the use of ab61153 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||1/50 - 1/100.|
|WB||1/500 - 1/1000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).|
FunctionATP-dependent RNA helicase. Acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. Also involved in HIV-1 replication. Interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location.
Sequence similaritiesBelongs to the DEAD box helicase family. DDX3/DED1 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Cellular localizationNucleus speckle. Cytoplasm. Located predominantly in nuclear speckles and, at low levels, throughout the cytoplasm. Located to the outer side of nuclear pore complexes (NPC). Shuttles between the nucleus and the cytoplasm in a XPO1-dependent manner.
- Information by UniProt
- ATP dependent RNA helicase DDX3X antibody
- ATP-dependent RNA helicase DDX3X antibody
- CAP Rf antibody
All lanes : Anti-DDX3 antibody (ab61153) at 1/500 dilution
Lane 1 : extracts from HepG2 cells
Lane 2 : extracts from HepG2 cells with immunizing peptide
Predicted band size: 73 kDa
Observed band size: 73 kDa
ab61153 at 1/50 dilution staining human brain tissue without (left) and with (right) blocking peptide; paraffin embedded.
ICC/IF image of ab61153 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61153, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Heaven MR et al. Composition of Rosenthal Fibers, the Protein Aggregate Hallmark of Alexander Disease. J Proteome Res 15:2265-82 (2016). Read more (PubMed: 27193225) »
- Ko C et al. DDX3 DEAD-box RNA helicase is a host factor that restricts hepatitis B virus replication at the transcriptional level. J Virol 88:13689-98 (2014). Read more (PubMed: 25231298) »