Anti-DDX3 antibody (ab62581)
Key features and details
- Rabbit polyclonal to DDX3
- Suitable for: ICC, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-DDX3 antibody
See all DDX3 primary antibodies -
Description
Rabbit polyclonal to DDX3 -
Host species
Rabbit -
Specificity
ab62581 detects both DDX3 and DBY. -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
A 16 amino acid peptide from near the center of human DDX3
-
Positive control
- HepG2 cells
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC |
Use a concentration of 10 µg/ml.
|
|
WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
|
Notes |
---|
ICC
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa). |
Target
-
Function
ATP-dependent RNA helicase. Acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. Also involved in HIV-1 replication. Interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location. -
Sequence similarities
Belongs to the DEAD box helicase family. DDX3/DED1 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Cellular localization
Nucleus speckle. Cytoplasm. Located predominantly in nuclear speckles and, at low levels, throughout the cytoplasm. Located to the outer side of nuclear pore complexes (NPC). Shuttles between the nucleus and the cytoplasm in a XPO1-dependent manner. - Information by UniProt
-
Database links
- Entrez Gene: 1654 Human
- Omim: 300160 Human
- SwissProt: O00571 Human
- Unigene: 380774 Human
-
Alternative names
- ATP dependent RNA helicase DDX3X antibody
- ATP-dependent RNA helicase DDX3X antibody
- CAP Rf antibody
see all
Images
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab62581 has been referenced in 2 publications.
- Li Q et al. DDX3X regulates cell survival and cell cycle during mouse early embryonic development. J Biomed Res 28:282-91 (2014). ICC/IF ; Mouse . PubMed: 25050112
- Soto-Rifo R et al. DEAD-box protein DDX3 associates with eIF4F to promote translation of selected mRNAs. EMBO J 31:3745-56 (2012). WB . PubMed: 22872150