Anti-DDX3 (phospho T322) antibody (ab61043)


  • Product name
    Anti-DDX3 (phospho T322) antibody
    See all DDX3 primary antibodies
  • Description
    Rabbit polyclonal to DDX3 (phospho T322)
  • Host species
  • Specificity
    Detects DDX3 only when phosphorylated at threonine 322
  • Tested applications
    Suitable for: ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from human DDX3 around the phosphorylation site of threonine 322 (V-A-TP-P-G).



Our Abpromise guarantee covers the use of ab61043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.


  • Function
    ATP-dependent RNA helicase. Acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. Also involved in HIV-1 replication. Interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location.
  • Sequence similarities
    Belongs to the DEAD box helicase family. DDX3/DED1 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localization
    Nucleus speckle. Cytoplasm. Located predominantly in nuclear speckles and, at low levels, throughout the cytoplasm. Located to the outer side of nuclear pore complexes (NPC). Shuttles between the nucleus and the cytoplasm in a XPO1-dependent manner.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP dependent RNA helicase DDX3X antibody
    • ATP-dependent RNA helicase DDX3X antibody
    • CAP Rf antibody
    • DBX antibody
    • DDX14 antibody
    • DDX3X antibody
    • DDX3X_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 3 X linked antibody
    • DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked antibody
    • DEAD box antibody
    • DEAD box protein 3 antibody
    • DEAD box protein 3 X-chromosomal antibody
    • DEAD box X isoform antibody
    • DEAD box, X isoform antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 3 antibody
    • DEAD/H box 3 antibody
    • DEAD/H box 3, X-linked antibody
    • Helicase like protein 2 antibody
    • Helicase-like protein 2 antibody
    • HLP2 antibody
    • X isoform antibody
    • X-chromosomal antibody
    see all


  • 1/500-1/1000 ab61043 staining phosphorilated DDX3 on HuvEc cells treated with 20% serum for 30 minutes, only in the absence of the immunizing phosphopeptide.


ab61043 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you very much for your answer. As my colleage is out of the office today, I am forwarding you already the answer from the laboratory in regards to the specificity of the ab61043. Please find the answer here below. I would like to reassure you aga...

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Thank you for taking time to complete our questionnaire. Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab61043 : 1) I would suggest to use the antibody in a tested application, ELISA or ICC/I...

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Thank you for contacting us. As far as we are aware, ab61043 has never been tested in western blot. All tested applications are specified on our datasheets, and these are updated as soon as any new information is brought to our attention. I am at...

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