Anti-DDX3 (phospho T322) antibody (ab61043)
Key features and details
- Rabbit polyclonal to DDX3 (phospho T322)
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DDX3 (phospho T322) antibody
See all DDX3 primary antibodies -
Description
Rabbit polyclonal to DDX3 (phospho T322) -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human DDX3 aa 450-550 (phospho T322).
Database link: O00571 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab61043 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/500 - 1/1000.
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Notes |
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ICC/IF
1/500 - 1/1000. |
Target
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Function
ATP-dependent RNA helicase. Acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. Also involved in HIV-1 replication. Interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location. -
Sequence similarities
Belongs to the DEAD box helicase family. DDX3/DED1 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Cellular localization
Nucleus speckle. Cytoplasm. Located predominantly in nuclear speckles and, at low levels, throughout the cytoplasm. Located to the outer side of nuclear pore complexes (NPC). Shuttles between the nucleus and the cytoplasm in a XPO1-dependent manner. - Information by UniProt
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Database links
- Entrez Gene: 1654 Human
- Entrez Gene: 13205 Mouse
- Entrez Gene: 317335 Rat
- Omim: 300160 Human
- SwissProt: O00571 Human
- SwissProt: Q62167 Mouse
- Unigene: 380774 Human
- Unigene: 289662 Mouse
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Alternative names
- ATP dependent RNA helicase DDX3X antibody
- ATP-dependent RNA helicase DDX3X antibody
- CAP Rf antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab61043 has been referenced in 1 publication.
- Brennan R et al. Investigating nucleo-cytoplasmic shuttling of the human DEAD-box helicase DDX3. Eur J Cell Biol 97:501-511 (2018). PubMed: 30131165