Overview

  • Product name
    Anti-DDX39 antibody [2252C4a]
    See all DDX39 primary antibodies
  • Description
    Mouse monoclonal [2252C4a] to DDX39
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IP, Dot blotmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment

  • Positive control
    • F2480 whole cell lysate. HeLa whole cell lysate. NIH3T3 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab50697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
Dot blot
  • Application notes
    Dot: Use at an assay dependent dilution.
    IP: Use at 5 µg.
    WB: 1/50 - 1/100. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX39 encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates 2 transcript variants encoding different isoforms.
    • Cellular localization
      Cytoplasmic and Nuclear
    • Database links
    • Alternative names
      • ATP dependent RNA helicase DDX39 antibody
      • ATP-dependent RNA helicase DDX39A antibody
      • BAT1 antibody
      • BAT1L antibody
      • DDX39 antibody
      • DDXL antibody
      • DEAD (Asp-Glu-Ala-Asp) box polypeptide 39 isoform 2 antibody
      • DEAD (Asp-Glu-Ala-Asp) box polypeptide 39A antibody
      • DEAD box protein 39 antibody
      • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 39 antibody
      • Eukaryotic initiation factor 4a antibody
      • Nuclear RNA helicase URH49 antibody
      • Nuclear RNA helicase, DECD variant of DEAD box family antibody
      • UAP56 related helicase antibody
      • UAP56 related helicase, 49 kDa antibody
      • URH49 antibody
      • wu:fc87b12 antibody
      • zgc:55881 antibody
      see all

    Images

    • Anti-DDX39 antibody [2252C4a] (ab50697) + immunized recombinant protein

      Predicted band size: 49 kDa
      Observed band size: 37 kDa
      why is the actual band size different from the predicted?

    • Anti-DDX39 antibody [2252C4a] (ab50697) at 1/100 dilution + HeLa whole cell lysate at 25 µg

      Secondary
      Mouse IgG antibody at 1/2500 dilution

      Developed using the ECL technique.

      Predicted band size: 49 kDa
      Observed band size: 49 kDa

    • 500µg HeLa cell lysate was prepared in RIPA buffer and immunoprecipitated with 5µg ab50697. The subsequent Western blot was probed with ab50697 at 1/50, followed by TrueBlot anti-mouse IgG at 1/1000. Developed by the ECL technique.
    • Anti-DDX39 antibody [2252C4a] (ab50697) at 1/100 dilution + NIH3T3 whole cell lysate at 25 µg

      Secondary
      Mouse IgG antibody at 1/2500 dilution

      Developed using the ECL technique.

      Predicted band size: 49 kDa
      Observed band size: 49 kDa

    • Anti-DDX39 antibody [2252C4a] (ab50697) at 1/100 dilution + F2480 whole cell lysate at 50 µg

      Secondary
      Mouse IgG antibody at 1/2500 dilution

      Developed using the ECL technique.

      Predicted band size: 49 kDa
      Observed band size: 49 kDa

    References

    ab50697 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HEK293)
    Permeabilization
    Yes - 0.3% Triton X-100 in Blocking Buffer
    Specification
    HEK293
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Feb 13 2018

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    30 µg
    Specification
    HEK293
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Dec 01 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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