Anti-DDX39 antibody [2252C4a] (ab50697)
Key features and details
- Mouse monoclonal [2252C4a] to DDX39
- Suitable for: WB, IP
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-DDX39 antibody [2252C4a]
See all DDX39 primary antibodies -
Description
Mouse monoclonal [2252C4a] to DDX39 -
Host species
Mouse -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human, Recombinant fragment -
Immunogen
Recombinant fragment
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Positive control
- WB: HeLa, NIH3T3, F2480 whole cell lysate. IP: HeLa cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 micrometer membrane. -
Clonality
Monoclonal -
Clone number
2252C4a -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab50697 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) | |
IP |
Notes |
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IP: Use at 5 µg.
WB: 1/50 - 1/100. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX39 encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates 2 transcript variants encoding different isoforms. -
Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 10212 Human
- Entrez Gene: 68278 Mouse
- SwissProt: O00148 Human
- SwissProt: Q8VDW0 Mouse
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Alternative names
- ATP dependent RNA helicase DDX39 antibody
- ATP-dependent RNA helicase DDX39A antibody
- BAT1 antibody
see all
Images
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Anti-DDX39 antibody [2252C4a] (ab50697) + immunized recombinant protein
Predicted band size: 49 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted? -
Anti-DDX39 antibody [2252C4a] (ab50697) at 1/100 dilution + HeLa whole cell lysate at 25 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 49 kDa
Observed band size: 49 kDa -
500µg HeLa cell lysate was prepared in RIPA buffer and immunoprecipitated with 5µg ab50697. The subsequent Western blot was probed with ab50697 at 1/50, followed by TrueBlot anti-mouse IgG at 1/1000. Developed by the ECL technique.
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Anti-DDX39 antibody [2252C4a] (ab50697) at 1/100 dilution + NIH3T3 whole cell lysate at 25 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 49 kDa
Observed band size: 49 kDa -
Anti-DDX39 antibody [2252C4a] (ab50697) at 1/100 dilution + F2480 whole cell lysate at 50 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 49 kDa
Observed band size: 49 kDa
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab50697 has been referenced in 1 publication.
- Kato M et al. DDX39 acts as a suppressor of invasion for bladder cancer. Cancer Sci 103:1363-9 (2012). PubMed: 22494014