Synthetic peptide within Human DDX39 aa 1-100 (N terminal) (Cysteine residue). The exact sequence is proprietary. Database link: O00148
WB: Human seminoma, Jurkat, HeLa, HepG2 and A431 cell lysates.
IHC-P: Human colon and kidney tissues.
ICC/IF: HepG2 cells.
IP: Jurkat cell lysate.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/50 - 1/100.
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX39 encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates 2 transcript variants encoding different isoforms.
Nuclear RNA helicase, DECD variant of DEAD box family antibody
UAP56 related helicase antibody
UAP56 related helicase, 49 kDa antibody
Western blot - Anti-DDX39 antibody [EPR13507(B)] (ab176348)
All lanes : Anti-DDX39 antibody [EPR13507(B)] (ab176348) at 1/10000 dilution
Lane 1 : Human seminoma lysate Lane 2 : Jurkat cell lysate Lane 3 : HeLa cell lysate Lane 4 : HepG2 cell lysate Lane 5 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 49 kDa
Western blot analysis on immunoprecipitation pellet from (1) Jurkat cells lysate or (2) 1X PBS (negative control) using ab176348 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Western blot analysis on immunoprecipitation pellet from (1) Jurkat cells lysate or (2) 1X PBS (negative control) using ab176348 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.