Overview

  • Product name
    Anti-DDX39 antibody [EPR13507(B)]
    See all DDX39 primary antibodies
  • Description
    Rabbit monoclonal [EPR13507(B)] to DDX39
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IP, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human DDX39 aa 1-100 (N terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: O00148

  • Positive control
    • WB: Human seminoma, Jurkat, HeLa, HepG2 and A431 cell lysates. IHC-P: Human colon and kidney tissues. ICC/IF: HepG2 cells. IP: Jurkat cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab176348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/50000. Predicted molecular weight: 49 kDa.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/50 - 1/100.

Target

  • Relevance
    DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX39 encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates 2 transcript variants encoding different isoforms.
  • Cellular localization
    Cytoplasmic and Nuclear
  • Database links
  • Alternative names
    • ATP dependent RNA helicase DDX39 antibody
    • ATP-dependent RNA helicase DDX39A antibody
    • BAT1 antibody
    • BAT1L antibody
    • DDX39 antibody
    • DDXL antibody
    • DEAD (Asp-Glu-Ala-Asp) box polypeptide 39 isoform 2 antibody
    • DEAD (Asp-Glu-Ala-Asp) box polypeptide 39A antibody
    • DEAD box protein 39 antibody
    • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 39 antibody
    • Eukaryotic initiation factor 4a antibody
    • Nuclear RNA helicase URH49 antibody
    • Nuclear RNA helicase, DECD variant of DEAD box family antibody
    • UAP56 related helicase antibody
    • UAP56 related helicase, 49 kDa antibody
    • URH49 antibody
    • wu:fc87b12 antibody
    • zgc:55881 antibody
    see all

Images

  • All lanes : Anti-DDX39 antibody [EPR13507(B)] (ab176348) at 1/10000 dilution

    Lane 1 : Human seminoma lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : HepG2 cell lysate
    Lane 5 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 49 kDa



    Western blot analysis on immunoprecipitation pellet from (1) Jurkat cells lysate or (2) 1X PBS (negative control) using ab176348 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling DDX39 with ab176348 at 1/50 dilution.

  • Immunofluorescent staining of HepG2 cells labeling DDX39 with ab176348 at 1/50 dilution.

  • Western blot analysis on immunoprecipitation pellet from (1) Jurkat cells lysate or (2) 1X PBS (negative control) using ab176348 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DDX39 with ab176348 at 1/50 dilution.

References

ab176348 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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