Anti-DDX39 antibody [EPR13507(B)] (ab176348)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-DDX39 antibody [EPR13507(B)]
See all DDX39 primary antibodies -
DescriptionRabbit monoclonal [EPR13507(B)] to DDX39
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IF, IP, Flow Cyt, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human DDX39 aa 1-100 (N terminal) (Cysteine residue). The exact sequence is proprietary.
Database link: O00148 -
Positive control
- WB: Human seminoma, Jurkat, HeLa, HepG2 and A431 cell lysates. IHC-P: Human colon and kidney tissues. ICC/IF: HepG2 cells. IP: Jurkat cell lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR13507(B)
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab176348 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/50000. Predicted molecular weight: 49 kDa. | |
| ICC/IF | 1/50 - 1/100. | |
| IP | 1/10 - 1/100. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IHC-P | 1/50 - 1/100. |
Target
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RelevanceDEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX39 encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates 2 transcript variants encoding different isoforms.
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Cellular localizationCytoplasmic and Nuclear
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Database links
- Entrez Gene: 10212 Human
- Entrez Gene: 68278 Mouse
- Entrez Gene: 89827 Rat
- SwissProt: O00148 Human
- SwissProt: Q8VDW0 Mouse
- SwissProt: Q5U216 Rat
- Unigene: 311609 Human
- Unigene: 28222 Mouse
see all -
Alternative names
- ATP dependent RNA helicase DDX39 antibody
- ATP-dependent RNA helicase DDX39A antibody
- BAT1 antibody
see all
Images
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![Western blot - Anti-DDX39 antibody [EPR13507(B)] (ab176348)](https://www.abcam.com/ps/products/176/ab176348/Images/ab176348-203780-WB2.jpg)
Western blot - Anti-DDX39 antibody [EPR13507(B)] (ab176348)All lanes : Anti-DDX39 antibody [EPR13507(B)] (ab176348) at 1/10000 dilution
Lane 1 : Human seminoma lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 49 kDaWestern blot analysis on immunoprecipitation pellet from (1) Jurkat cells lysate or (2) 1X PBS (negative control) using ab176348 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX39 antibody [EPR13507(B)] (ab176348)](https://www.abcam.com/ps/products/176/ab176348/Images/ab176348-203781-IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX39 antibody [EPR13507(B)] (ab176348)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling DDX39 with ab176348 at 1/50 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-DDX39 antibody [EPR13507(B)] (ab176348)](https://www.abcam.com/ps/products/176/ab176348/Images/ab176348-203783-ICC.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DDX39 antibody [EPR13507(B)] (ab176348)Immunofluorescent staining of HepG2 cells labeling DDX39 with ab176348 at 1/50 dilution.
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![Immunoprecipitation - Anti-DDX39 antibody [EPR13507(B)] (ab176348)](https://www.abcam.com/ps/products/176/ab176348/Images/ab176348-203785-IP.jpg)
Immunoprecipitation - Anti-DDX39 antibody [EPR13507(B)] (ab176348)Western blot analysis on immunoprecipitation pellet from (1) Jurkat cells lysate or (2) 1X PBS (negative control) using ab176348 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX39 antibody [EPR13507(B)] (ab176348)](https://www.abcam.com/ps/products/176/ab176348/Images/ab176348-203782-IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX39 antibody [EPR13507(B)] (ab176348)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DDX39 with ab176348 at 1/50 dilution.
Datasheets and documents
Protocols
References
ab176348 has not yet been referenced specifically in any publications.
