Recombinant
RabMAb

Recombinant Anti-DDX39 antibody [EPR13507(B)] - BSA and Azide free (ab236015)

Overview

  • Product name

    Anti-DDX39 antibody [EPR13507(B)] - BSA and Azide free
    See all DDX39 primary antibodies
  • Description

    Rabbit monoclonal [EPR13507(B)] to DDX39 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, ICC/IF, Flow Cyt, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human DDX39 aa 1-100 (N terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: O00148

  • Positive control

    • IHC-P: Human colon tissue.
  • General notes

    Ab236015 is the carrier-free version of ab176348. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab236015 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab236015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB Use at an assay dependent concentration. Predicted molecular weight: 49 kDa.
IHC-P Use at an assay dependent concentration.

Target

  • Relevance

    DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX39 encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates 2 transcript variants encoding different isoforms.
  • Cellular localization

    Cytoplasmic and Nuclear
  • Database links

  • Alternative names

    • ATP dependent RNA helicase DDX39 antibody
    • ATP-dependent RNA helicase DDX39A antibody
    • BAT1 antibody
    • BAT1L antibody
    • DDX39 antibody
    • DDXL antibody
    • DEAD (Asp-Glu-Ala-Asp) box polypeptide 39 isoform 2 antibody
    • DEAD (Asp-Glu-Ala-Asp) box polypeptide 39A antibody
    • DEAD box protein 39 antibody
    • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 39 antibody
    • Eukaryotic initiation factor 4a antibody
    • Nuclear RNA helicase URH49 antibody
    • Nuclear RNA helicase, DECD variant of DEAD box family antibody
    • UAP56 related helicase antibody
    • UAP56 related helicase, 49 kDa antibody
    • URH49 antibody
    • wu:fc87b12 antibody
    • zgc:55881 antibody
    see all

Images

  • Western blot analysis on immunoprecipitation pellet from (1) Jurkat cells lysate or (2) 1X PBS (negative control) using ab176348 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176348).

  • Immunofluorescent staining of HepG2 cells labeling DDX39 with ab176348 at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176348).

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DDX39 with ab176348 at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176348).

  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling DDX39 with ab176348 at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176348).

References

ab236015 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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