Overview

  • Product name

    Anti-DDX39 antibody [EPR13508]
    See all DDX39 primary antibodies
  • Description

    Rabbit monoclonal [EPR13508] to DDX39
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WBmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human DDX39 aa 350 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: O00148

  • Positive control

    • Jurkat, A431, HeLa, HepG2, and Human seminoma lysates. Human breast and colon tissue. Permabilized Jurkat cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab180857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/5000 - 1/1000. Predicted molecular weight: 49 kDa.
  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Relevance

      DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX39 encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates 2 transcript variants encoding different isoforms.
    • Cellular localization

      Cytoplasmic and Nuclear
    • Database links

    • Alternative names

      • ATP dependent RNA helicase DDX39 antibody
      • ATP-dependent RNA helicase DDX39A antibody
      • BAT1 antibody
      • BAT1L antibody
      • DDX39 antibody
      • DDXL antibody
      • DEAD (Asp-Glu-Ala-Asp) box polypeptide 39 isoform 2 antibody
      • DEAD (Asp-Glu-Ala-Asp) box polypeptide 39A antibody
      • DEAD box protein 39 antibody
      • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 39 antibody
      • Eukaryotic initiation factor 4a antibody
      • Nuclear RNA helicase URH49 antibody
      • Nuclear RNA helicase, DECD variant of DEAD box family antibody
      • UAP56 related helicase antibody
      • UAP56 related helicase, 49 kDa antibody
      • URH49 antibody
      • wu:fc87b12 antibody
      • zgc:55881 antibody
      see all

    Images

    • All lanes : Anti-DDX39 antibody [EPR13508] (ab180857) at 1/5000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : HepG2 cell lysate
      Lane 5 : Human seminoma lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 49 kDa

    • Immunohistochemical analysis of Human breast tissue, staining DDX39 with ab180857 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of Human colon tissue, staining DDX39 with ab180857 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow cytometry analysis of DDX39 expression in permeabilized Jurkat cells using ab180857 at 1/10 dilution (red) and a rabbit IgG as negative control (green).

    References

    This product has been referenced in:

    • Dobrotkova V  et al. Prediction of neuroblastoma cell response to treatment with natural or synthetic retinoids using selected protein biomarkers. PLoS One 14:e0218269 (2019). Read more (PubMed: 31188873) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab180857.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up