Recombinant Anti-DDX39 antibody [EPR13508] (ab180857)
![Western blot - Anti-DDX39 antibody [EPR13508] (ab180857)](https://www.abcam.com/ps/products/180/ab180857/Images/ab180857-210153-anti-ddx39-antibody-epr13508-western-blot.jpg "Western blot - Anti-DDX39 antibody [EPR13508] (ab180857)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13508] to DDX39
- Suitable for: Flow Cyt, IHC-P, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-DDX39 antibody [EPR13508]
See all DDX39 primary antibodies -
Description
Rabbit monoclonal [EPR13508] to DDX39 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IHC-P, WBmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human DDX39 aa 350 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
Database link: O00148 -
Positive control
- Jurkat, A431, HeLa, HepG2, and Human seminoma lysates. Human breast and colon tissue. Permabilized Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13508 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab180857 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/5000 - 1/1000. Predicted molecular weight: 49 kDa. |
Target
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Relevance
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX39 encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates 2 transcript variants encoding different isoforms. -
Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 10212 Human
- Entrez Gene: 68278 Mouse
- Entrez Gene: 89827 Rat
- SwissProt: O00148 Human
- SwissProt: Q8VDW0 Mouse
- SwissProt: Q5U216 Rat
- Unigene: 311609 Human
- Unigene: 28222 Mouse
see all -
Alternative names
- ATP dependent RNA helicase DDX39 antibody
- ATP-dependent RNA helicase DDX39A antibody
- BAT1 antibody
see all
Images
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Western blot - Anti-DDX39 antibody [EPR13508] (ab180857)All lanes : Anti-DDX39 antibody [EPR13508] (ab180857) at 1/5000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : Human seminoma lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 49 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX39 antibody [EPR13508] (ab180857)](https://www.abcam.com/ps/products/180/ab180857/Images/ab180857-210155-anti-ddx39-antibody-epr13508-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX39 antibody [EPR13508] (ab180857)Immunohistochemical analysis of Human breast tissue, staining DDX39 with ab180857 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX39 antibody [EPR13508] (ab180857)](https://www.abcam.com/ps/products/180/ab180857/Images/ab180857-210156-anti-ddx39-antibody-epr13508-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX39 antibody [EPR13508] (ab180857)Immunohistochemical analysis of Human colon tissue, staining DDX39 with ab180857 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Flow Cytometry - Anti-DDX39 antibody [EPR13508] (ab180857)](https://www.abcam.com/ps/products/180/ab180857/Images/ab180857-210157-anti-ddx39-antibody-epr13508-flow-cytometry.jpg)
Flow Cytometry - Anti-DDX39 antibody [EPR13508] (ab180857)Flow cytometry analysis of DDX39 expression in permeabilized Jurkat cells using ab180857 at 1/10 dilution (red) and a rabbit IgG as negative control (green).
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![Anti-DDX39 antibody [EPR13508] (ab180857)](https://www.abcam.com/ps/products/180/ab180857/Images/ab180857-6-benefits-of-recombinant-antibodies.png)
Anti-DDX39 antibody [EPR13508] (ab180857)
Protocols
Datasheets and documents
Certificate of Compliance
References (1)
ab180857 has been referenced in 1 publication.
- Dobrotkova V et al. Prediction of neuroblastoma cell response to treatment with natural or synthetic retinoids using selected protein biomarkers. PLoS One 14:e0218269 (2019). PubMed: 31188873
