• Product name
  • Description
    Rabbit polyclonal to DDX3Y
  • Host species
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a sequence within amino acids 70-316 of Human DDX3Y.

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2, Molt4, and Raji cells and whole cell lysates.



Our Abpromise guarantee covers the use of ab95979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 73 kDa.
ICC/IF 1/100 - 1/200.


  • Function
    Probable ATP-dependent RNA helicase. May play a role in spermatogenesis.
  • Tissue specificity
    Testis-specific. Expressed predominantly in spermatogonia.
  • Involvement in disease
    Note=DDX3Y is located in the 'azoospermia factor a' (AZFa) region on chromosome Y which is deleted in Sertoli cell-only syndrome. This is an infertility disorder in which no germ cells are visible in seminiferous tubules leading to azoospermia.
  • Sequence similarities
    Belongs to the DEAD box helicase family. DDX3/DED1 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localization
    Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm in an XPO1-dependent manner.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP dependent RNA helicase DDX3Y antibody
    • ATP-dependent RNA helicase DDX3Y antibody
    • DBY antibody
    • DDX3Y antibody
    • DDX3Y_HUMAN antibody
    • DEAD (Asp-Glu-Ala-Asp) box polypeptide 3 Y linked antibody
    • DEAD box protein 3 Y chromosomal antibody
    • DEAD box protein 3 antibody
    • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide Y chromosome antibody
    • Y-chromosomal antibody
    see all


  • All lanes : Anti-DDX3Y antibody (ab95979) at 1/10000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 73 kDa

  • ab95979 at 1/200 dilution staining DDX3Y in HeLa cells by Immunofluorescence, Paraformaldehyde-fixed. Lower image is merged with DNA probe.


ab95979 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us.

The lab has confirmed that the immunogen for ab95979 shares 96% identity with DDX3X, so it has high chance of cross-reacting with DDX3X which is why it is cross reacting with Hela cells.

I hope this inform...

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Thank you for contacting us.

One possible reason of this cross reactivity is due to the sequence homology between DDX3Y and DDX3X. Please click the link below to see the DDX3Y sequence alignment with DDX3X; the similarity is 92% which is more ...

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Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future or...

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Thank you for contacting Abcam regarding ab95979.

I am sorry that the customer has experienced difficulties with this antibody in IF. I reviewed the protocol details provided and I would just like to clarify if the customer is using a h...

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