Overview

  • Product name

    Anti-DDX4 / MVH antibody [EPR21785]
    See all DDX4 / MVH primary antibodies
  • Description

    Rabbit monoclonal [EPR21785] to DDX4 / MVH
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human DDX4/ MVH aa 50-150. The exact sequence is proprietary.
    Database link: Q9NQI0

  • Positive control

    • WB: Human testis lysate. IHC-P: Human testis and ovary tissues.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab235443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).

Target

  • Function

    Probable ATP-dependent RNA helicase required during spermatogenesis (PubMed:10920202, PubMed:21034600). Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle.
  • Tissue specificity

    Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes.
  • Sequence similarities

    Belongs to the DEAD box helicase family. DDX4/VASA subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localization

    Cytoplasm. Cytoplasm, perinuclear region. Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis.
  • Information by UniProt
  • Database links

  • Alternative names

    • DDX 4 antibody
    • Ddx4 antibody
    • DDX4_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 4 antibody
    • DEAD box protein 4 antibody
    • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4 antibody
    • MVH antibody
    • Probable ATP dependent RNA helicase DDX4 antibody
    • Probable ATP-dependent RNA helicase DDX4 antibody
    • VASA antibody
    • Vasa homolog antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human ovary tissue labeling DDX4 / MVH with ab235443 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in oocytes of human ovary (PMID:10920202; PMID:26444630). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue labeling DDX4 / MVH with ab235443 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in germ cells of human testis (PMID: 10920202; PMID: 26444630). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-DDX4 / MVH antibody [EPR21785] (ab235443) at 1/1000 dilution + Human testis lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 79 kDa
    Observed band size: 79 kDa


    Exposure time: 6 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 26482643).

References

ab235443 has not yet been referenced specifically in any publications.

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