Recombinant Anti-DDX41 antibody [EPR14298] (ab182007)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14298] to DDX41
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-DDX41 antibody [EPR14298]
See all DDX41 primary antibodies -
Description
Rabbit monoclonal [EPR14298] to DDX41 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, Jurkat, A549 or Jar lysate, Human colon tissue or HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14298 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182007 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
For antigen retrieval, we recommend Heat Induced Epitope Retrieval (HIER) by using Decloaking Chamber/Pressure Cooker. Run the heat program for 30 seconds at 125°C, then let the slides cool down to room temperature. |
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 70 kDa.
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ICC/IF |
1/250.
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Notes |
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IHC-P
1/50. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. For antigen retrieval, we recommend Heat Induced Epitope Retrieval (HIER) by using Decloaking Chamber/Pressure Cooker. Run the heat program for 30 seconds at 125°C, then let the slides cool down to room temperature. |
WB
1/1000 - 1/10000. Predicted molecular weight: 70 kDa. |
ICC/IF
1/250. |
Target
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Function
Probable ATP-dependent RNA helicase. Is required during post-transcriptional gene expression. May be involved in pre-mRNA splicing. -
Sequence similarities
Belongs to the DEAD box helicase family. DDX41 subfamily.
Contains 1 CCHC-type zinc finger.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 51428 Human
- Entrez Gene: 72935 Mouse
- Entrez Gene: 314336 Rat
- Omim: 608170 Human
- SwissProt: Q9UJV9 Human
- SwissProt: Q91VN6 Mouse
- Unigene: 484288 Human
- Unigene: 205045 Mouse
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Alternative names
- 2900024F02Rik antibody
- AA958953 antibody
- ABS antibody
see all
Images
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Anti-DDX41 antibody [EPR14298] (ab182007) at 1/10000 dilution + Jar cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 70 kDa -
Immunofluorescence analysis of HeLa cells (fixative -20℃ Acetone) labeling DDX41 with ab182007 at a 1/250 dilution. Goat anti rabbit IgG (Dylight 488) secondary used at a 1/200 diution.
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Immunohistochemical analysis of paraffin embedded Human colon tissue labeling DDX41 with ab182007 at a 1/50 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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All lanes : Anti-DDX41 antibody [EPR14298] (ab182007) at 1/2000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293 cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 70 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab182007 has been referenced in 1 publication.
- Kadono M et al. Biological implications of somatic DDX41 p.R525H mutation in acute myeloid leukemia. Exp Hematol 44:745-754.e4 (2016). PubMed: 27174803