Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14298] to DDX41
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Product nameAnti-DDX41 antibody [EPR14298]
See all DDX41 primary antibodies
DescriptionRabbit monoclonal [EPR14298] to DDX41
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DDX41 aa 600 to the C-terminus. The exact sequence is proprietary.
Database link: Q9UJV9
- HeLa, Jurkat, A549 or Jar lysate, Human colon tissue or HeLa cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab182007 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
For antigen retrieval, we recommend Heat Induced Epitope Retrieval (HIER) by using Decloaking Chamber/Pressure Cooker. Run the heat program for 30 seconds at 125°C, then let the slides cool down to room temperature.
|WB||1/1000 - 1/10000. Predicted molecular weight: 70 kDa.|
FunctionProbable ATP-dependent RNA helicase. Is required during post-transcriptional gene expression. May be involved in pre-mRNA splicing.
Sequence similaritiesBelongs to the DEAD box helicase family. DDX41 subfamily.
Contains 1 CCHC-type zinc finger.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
- Information by UniProt
- 2900024F02Rik antibody
- AA958953 antibody
- ABS antibody
Anti-DDX41 antibody [EPR14298] (ab182007) at 1/10000 dilution + Jar cell lysate at 20 µg
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 70 kDa
Immunofluorescence analysis of HeLa cells (fixative -20℃ Acetone) labeling DDX41 with ab182007 at a 1/250 dilution. Goat anti rabbit IgG (Dylight 488) secondary used at a 1/200 diution.
Immunohistochemical analysis of paraffin embedded Human colon tissue labeling DDX41 with ab182007 at a 1/50 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
All lanes : Anti-DDX41 antibody [EPR14298] (ab182007) at 1/2000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293 cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 70 kDa
ab182007 has not yet been referenced specifically in any publications.