Key features and details
- Rabbit polyclonal to DDX46
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-DDX46 antibody
See all DDX46 primary antibodies
DescriptionRabbit polyclonal to DDX46
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus
Synthetic peptide corresponding to a region between residue 981 and the C-terminus (residue 1031) of human DDX46 (NP_055644.2)
- Whole cell lysate from HeLa, 293T or NIH3T3 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72083 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 117 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevancePlays an essential role in splicing, either prior to, or during splicing A complex formation.
Cellular localizationNucleus speckle. Nucleus, Cajal body.
- DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 antibody
- DEAD box protein 46 antibody
- Probable ATP dependent RNA helicase DDX46 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling DDX46 with ab72083 at 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-DDX46 antibody (ab72083) at 0.04 µg/ml
Lane 1 : Whole cell
lysate from HeLa cells at 50 µg
Lane 2 : Whole cell
lysate from HeLa cells at 15 µg
Lane 3 : Whole cell
lysate from HeLa cells at 5 µg
Lane 4 : Whole cell
lysate from 293T cells at 50 µg
Lane 5 : Whole cell
lysate from NIH 3T3 cells at 50 µg
Predicted band size: 117 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Additional bands at: 75 kDa, 77 kDa. We are unsure as to the identity of these extra bands.
Analysis of Human DDX46 by Immunoprecipitation in whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded) cells using ab72083 at 3 µg/mg lysate (lane 1) or a control rabbit IgG (lane 2). ab72083 was used for WB detection at 1 µg/ml. Detection: chemioluminescence, exposure time 3 seconds.
ab72083 has been referenced in 1 publication.
- Zheng Q et al. The RNA helicase DDX46 inhibits innate immunity by entrapping m6A-demethylated antiviral transcripts in the nucleus. Nat Immunol 18:1094-1103 (2017). WB . PubMed: 28846086