Overview

  • Product name

  • Description

    Rabbit polyclonal to DDX47
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Purified Human DDX47 with proprietary tag.

  • Positive control

    • HeLa whole cell extratcs; HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% Whole serum
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab128204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 50 kDa.
ICC/IF 1/500.

Target

Images

  • Anti-DDX47 antibody (ab128204) at 1/1000 dilution + HeLa whole cell extract at 10 µg

    Predicted band size: 50 kDa

  • ab128204, at 1/500, staining DDX47 in HeLa cells by Immunoflouroscence. FITC-conjugated anti-rabbit IgG was used as secondary antibody. DNA was stained with Hoechst dye.

References

This product has been referenced in:

  • Sekiguchi T  et al. NOP132 is required for proper nucleolus localization of DEAD-box RNA helicase DDX47. Nucleic Acids Res 34:4593-608 (2006). Read more (PubMed: 16963496) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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