Product nameAnti-DDX47 antibody
See all DDX47 primary antibodies
DescriptionRabbit polyclonal to DDX47
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Gorilla, Orangutan
- HeLa and 293T whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab176852 was affinity purified using an epitope specific to DDX47 immobilized on solid support.
Our Abpromise guarantee covers the use of ab176852 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 50 kDa.|
Sequence similaritiesBelongs to the DEAD box helicase family. DDX47/RRP3 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- DDX47 antibody
- DDX47_HUMAN antibody
- DEAD (Asp Glu Ala Asp) box polypeptide 47 antibody
All lanes : Anti-DDX47 antibody (ab176852) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 50 kDa
Exposure time: 3 minutes
Ab176852 immunoprecipitating DDX47 in HeLa whole cell lysate. 3µg/mg of cell lysate was incubated with primary antibody.
ab176852 has not yet been referenced specifically in any publications.