Product nameAnti-DDX47 antibody
See all DDX47 primary antibodies
DescriptionRabbit polyclonal to DDX47
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Cow, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Chinese hamster
Synthetic peptide within Human DDX47 aa 405-455. The exact sequence is proprietary. (NP_057439.2).
Database link: Q9H0S4
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab225870 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 50 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
Sequence similaritiesBelongs to the DEAD box helicase family. DDX47/RRP3 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- DDX47 antibody
- DDX47_HUMAN antibody
- DEAD (Asp Glu Ala Asp) box polypeptide 47 antibody
All lanes : Anti-DDX47 antibody (ab225870) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 50 kDa
Exposure time: 3 minutes
Lysates prepared using NETN lysis buffer.
DDX47 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225870 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225870 at 0.4 µg/ml.
Lane 1: ab225870 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
ab225870 has not yet been referenced specifically in any publications.