Overview

  • Product name

  • Description

    Mouse polyclonal to DDX5
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Full-length Human DDX5 protein (AAH16027)

  • Positive control

    • DDX5 transfected 293T lysate, Hela nuclear extract, Raw 264.7 cell lysate and Hela cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab92917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notes
    ICC/IF: Use at a concentration of 10 µg/ml.
    WB: 1/500 - 1/1000. Predicted molecular weight: 69 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      RNA-dependent ATPase activity. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. May be involved in pre-mRNA splicing.
    • Sequence similarities

      Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • Post-translational
      modifications

      Arg-502 is dimethylated, probably to asymmetric dimethylarginine.
    • Cellular localization

      Nucleus > nucleolus.
    • Information by UniProt
    • Database links

    • Alternative names

      • ATP dependent RNA helicase DDX5 antibody
      • DDX 5 antibody
      • Ddx5 antibody
      • DDX5_HUMAN antibody
      • DEAD (Asp Glu Ala Asp) box helicase 5 antibody
      • DEAD (Asp Glu Ala Asp) box polypeptide 5 antibody
      • DEAD box 5 antibody
      • DEAD box protein 5 antibody
      • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 5 (RNA helicase, 68kD) antibody
      • G17P1 antibody
      • HELR antibody
      • HLR1 antibody
      • HUMP68 antibody
      • P68 antibody
      • p68 RNA helicase antibody
      • Probable ATP dependent RNA helicase DDX5 antibody
      • Probable ATP-dependent RNA helicase DDX5 antibody
      • RNA helicase p68 antibody
      see all

    Images

    • Anti-DDX5 antibody (ab92917) at 1/500 dilution + Hela nuclear extract at 50 µg

      Predicted band size: 69 kDa

    • Anti-DDX5 antibody (ab92917) at 1/500 dilution + Mouse raw 264.7 cell lysate at 50 µg

      Predicted band size: 69 kDa

    • All lanes : Anti-DDX5 antibody (ab92917) at 1/500 dilution

      Lane 1 : DDX5 (human) transfected lysate
      Lane 2 : Non-transfected lysate

      Lysates/proteins at 25 µg per lane.

      Predicted band size: 69 kDa
      Observed band size: 68 kDa
      why is the actual band size different from the predicted?

    • Immunofluorescence analysis of HeLa cells labelled with ab92917 at 10 µg/ml.

    References

    ab92917 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab92917.
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