Anti-DDX5 antibody (ab92917)
Key features and details
- Mouse polyclonal to DDX5
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-DDX5 antibody
See all DDX5 primary antibodies -
Description
Mouse polyclonal to DDX5 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein within Human DDX5. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- DDX5 transfected 293T lysate, Hela nuclear extract, Raw 264.7 cell lysate and Hela cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 69 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 69 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
RNA-dependent ATPase activity. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. May be involved in pre-mRNA splicing. -
Sequence similarities
Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Post-translational
modificationsArg-502 is dimethylated, probably to asymmetric dimethylarginine. -
Cellular localization
Nucleus > nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 1655 Human
- Entrez Gene: 13207 Mouse
- Omim: 180630 Human
- SwissProt: P17844 Human
- SwissProt: Q61656 Mouse
- Unigene: 279806 Human
- Unigene: 220038 Mouse
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Alternative names
- ATP dependent RNA helicase DDX5 antibody
- DDX 5 antibody
- Ddx5 antibody
see all
Images
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Anti-DDX5 antibody (ab92917) at 1/500 dilution + Hela nuclear extract at 50 µg
Predicted band size: 69 kDa -
Anti-DDX5 antibody (ab92917) at 1/500 dilution + Mouse raw 264.7 cell lysate at 50 µg
Predicted band size: 69 kDa -
All lanes : Anti-DDX5 antibody (ab92917) at 1/500 dilution
Lane 1 : DDX5 (human) transfected lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 69 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted? -
Immunofluorescence analysis of HeLa cells labelled with ab92917 at 10 µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab92917 has not yet been referenced specifically in any publications.