Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-DDX5 antibody [EPR7239] (ab126730)

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Overview

  • Product name

    Anti-DDX5 antibody [EPR7239]
    See all DDX5 primary antibodies

  • Description

    Rabbit monoclonal [EPR7239] to DDX5

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide, corresponding to residues in Human DDX5 (UniProt P17844).

  • Positive control

    • HeLa, SH-SY5Y, A431, and HT29 cell lysates; Human bladder carcinoma and Human kidney tissues; HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR7239

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 69 kDa).
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.
ICC/IF 1/100 - 1/250.

Target

  • Function

    RNA-dependent ATPase activity. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. May be involved in pre-mRNA splicing.

  • Sequence similarities

    Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.

  • Post-translational
    modifications

    Arg-502 is dimethylated, probably to asymmetric dimethylarginine.

  • Cellular localization

    Nucleus > nucleolus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ATP dependent RNA helicase DDX5 antibody
    • DDX 5 antibody
    • Ddx5 antibody
    • DDX5_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box helicase 5 antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 5 antibody
    • DEAD box 5 antibody
    • DEAD box protein 5 antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 5 (RNA helicase, 68kD) antibody
    • G17P1 antibody
    • HELR antibody
    • HLR1 antibody
    • HUMP68 antibody
    • P68 antibody
    • p68 RNA helicase antibody
    • Probable ATP dependent RNA helicase DDX5 antibody
    • Probable ATP-dependent RNA helicase DDX5 antibody
    • RNA helicase p68 antibody
    see all

Images

  • Western blot - Anti-DDX5 antibody [EPR7239] (ab126730)
    Western blot - Anti-DDX5 antibody [EPR7239] (ab126730)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: DDX5 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: MCF7 whole cell lysate (20 µg)
    Lane 4: HeLa whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab126730 observed at 69 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab126730 was shown to specifically react with DDX5 in wild-type cells as signal was lost in DDX5 knockout cells. Wild-type and DDX5 knockout samples were subjected to SDS-PAGE. Ab126730 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-DDX5 antibody [EPR7239] (ab126730)
    Flow Cytometry - Anti-DDX5 antibody [EPR7239] (ab126730)

    ab126730 staining DDX5 in the human cell line HeLa (Human cervix adenocarcinoma epithelial cell) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permeabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/200. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black) Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue).

  • Western blot - Anti-DDX5 antibody [EPR7239] (ab126730)
    Western blot - Anti-DDX5 antibody [EPR7239] (ab126730)
    All lanes : Anti-DDX5 antibody [EPR7239] (ab126730) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : HT29 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 69 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-DDX5 antibody [EPR7239] (ab126730)
    Immunocytochemistry/ Immunofluorescence - Anti-DDX5 antibody [EPR7239] (ab126730)

    Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y (human neuroblastoma) cells labelling DDX5 (green) with purified ab126730 at 1/250. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as a nuclear counterstain.  

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX5 antibody [EPR7239] (ab126730)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX5 antibody [EPR7239] (ab126730)

    ab126730, at a dilution of 1/250, staining DDX5 in paraffin-embedded Human bladder carcinoma tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX5 antibody [EPR7239] (ab126730)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX5 antibody [EPR7239] (ab126730)

    ab126730, at a dilution of 1/250, staining DDX5 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-DDX5 antibody [EPR7239] (ab126730)
    Immunocytochemistry/ Immunofluorescence - Anti-DDX5 antibody [EPR7239] (ab126730)
    ab126730, at a dilution of 1/100, staining DDX5 in HeLa cells by Immunofluorescence.

References

This product has been referenced in:

  • Hoffmann N  et al. DGCR8 Promotes Neural Progenitor Expansion and Represses Neurogenesis in the Mouse Embryonic Neocortex. Front Neurosci 12:281 (2018). Read more (PubMed: 29760646) »
  • Dai L  et al. High expression of ALDOA and DDX5 are associated with poor prognosis in human colorectal cancer. Cancer Manag Res 10:1799-1806 (2018). Read more (PubMed: 29988738) »
See all 5 Publications for this product

Publishing research using ab126730? Please let us know so that we can cite the reference in this datasheet.

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