Recombinant Anti-DDX5 antibody [EPR7239] - BSA and Azide free (ab240028)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7239] to DDX5 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, IP, WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
-
Product name
Anti-DDX5 antibody [EPR7239] - BSA and Azide free
See all DDX5 primary antibodies -
Description
Rabbit monoclonal [EPR7239] to DDX5 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- IP: Mouse brain lysate.
-
General notes
ab240028 is the carrier-free version of ab126730.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7239 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
- Anti-DDX5 antibody [EPR7239] (ab126730)
- Alexa Fluor® 488 Anti-DDX5 antibody [EPR7239] (ab199226)
- Alexa Fluor® 647 Anti-DDX5 antibody [EPR7239] (ab199460)
- PE Anti-DDX5 antibody [EPR7239] (ab305889)
- APC Anti-DDX5 antibody [EPR7239] (ab305890)
- HRP Anti-DDX5 antibody [EPR7239] (ab305891)
- Alexa Fluor® 594 Anti-DDX5 antibody [EPR7239] (ab310580)
- Alexa Fluor® 555 Anti-DDX5 antibody [EPR7239] (ab312109)
- Alexa Fluor® 568 Anti-DDX5 antibody [EPR7239] (ab312592)
-
Compatible Secondaries
-
Conjugation kits
-
Immunohistochemistry kits
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab240028 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
Use at an assay dependent concentration.
|
|
ICC/IF |
Use at an assay dependent concentration.
|
|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
IP |
Use at an assay dependent concentration.
|
|
WB |
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 69 kDa).
|
Notes |
---|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 69 kDa). |
Target
-
Function
RNA-dependent ATPase activity. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. May be involved in pre-mRNA splicing. -
Sequence similarities
Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Post-translational
modificationsArg-502 is dimethylated, probably to asymmetric dimethylarginine. -
Cellular localization
Nucleus > nucleolus. - Information by UniProt
-
Database links
- Entrez Gene: 1655 Human
- Entrez Gene: 13207 Mouse
- Entrez Gene: 287765 Rat
- Omim: 180630 Human
- SwissProt: P17844 Human
- SwissProt: Q61656 Mouse
- Unigene: 279806 Human
- Unigene: 220038 Mouse
-
Alternative names
- ATP dependent RNA helicase DDX5 antibody
- DDX 5 antibody
- Ddx5 antibody
see all
Images
-
ab126730 staining DDX5 in the human cell line HeLa (Human cervix adenocarcinoma epithelial cell) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permeabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/200. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black) Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126730).
-
Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y (human neuroblastoma) cells labelling DDX5 (green) with purified ab126730 at 1/250. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as a nuclear counterstain.
Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126730).
-
ab126730, at a dilution of 1/250, staining DDX5 in paraffin-embedded Human bladder carcinoma tissue by Immunohistochemistry.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126730).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Purified ab126730 at 1/20 dilution (1µg) immunoprecipitating DDX5 in Mouse brain lysate.
Lane 1 (input): Mouse brain lysate 10µg
Lane 2 (+): ab126730 + Mouse brain lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab126730 in Mouse brain lysate.
VeriBlot for IP Detection Reagent (HRP)(ab131366) (1/20,000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 69 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126730). -
ab126730, at a dilution of 1/250, staining DDX5 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126730).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab240028 has not yet been referenced specifically in any publications.