Overview

  • Product name

    Anti-DDX5 antibody [EPR7240]
    See all DDX5 primary antibodies
  • Description

    Rabbit monoclonal [EPR7240] to DDX5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human DDX5 aa 550-650. The exact sequence is proprietary.

  • Positive control

    • HT29 and SHSY5Y cell lysates; Human breast carcinoma and colon tissues; SHSY5Y cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7240
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab128928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 69 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      RNA-dependent ATPase activity. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. May be involved in pre-mRNA splicing.
    • Sequence similarities

      Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • Post-translational
      modifications

      Arg-502 is dimethylated, probably to asymmetric dimethylarginine.
    • Cellular localization

      Nucleus > nucleolus.
    • Information by UniProt
    • Database links

    • Alternative names

      • ATP dependent RNA helicase DDX5 antibody
      • DDX 5 antibody
      • Ddx5 antibody
      • DDX5_HUMAN antibody
      • DEAD (Asp Glu Ala Asp) box helicase 5 antibody
      • DEAD (Asp Glu Ala Asp) box polypeptide 5 antibody
      • DEAD box 5 antibody
      • DEAD box protein 5 antibody
      • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 5 (RNA helicase, 68kD) antibody
      • G17P1 antibody
      • HELR antibody
      • HLR1 antibody
      • HUMP68 antibody
      • P68 antibody
      • p68 RNA helicase antibody
      • Probable ATP dependent RNA helicase DDX5 antibody
      • Probable ATP-dependent RNA helicase DDX5 antibody
      • RNA helicase p68 antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: DDX5 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: MCF7 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab128928 observed at 69 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab128928 was shown to specifically react with DDX5 in wild-type cells as signal was lost in DDX5 knockout cells. Wild-type and DDX5 knockout samples were subjected to SDS-PAGE. Ab128928 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-DDX5 antibody [EPR7240] (ab128928) at 1/1000 dilution

      Lane 1 : HT29 cell lysate
      Lane 2 : SHSY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 69 kDa

    • ab128928 at 1/100 dilution staining DDX5 in paraffin-embedded Human breast carcinoma tissue by immunohistochemistry.
    • ab128928 at 1/100 dilution staining DDX5 in paraffin-embedded Human colon tissue by immunohistochemistry.
    • ab128928 at 1/100 dilution staining DDX5 in SHSY5Y cells by immunofluorescence.

    References

    This product has been referenced in:

    • Jiang FZ  et al. Mutant p53 induces EZH2 expression and promotes epithelial-mesenchymal transition by disrupting p68-Drosha complex assembly and attenuating miR-26a processing. Oncotarget 6:44660-74 (2015). IP . Read more (PubMed: 26587974) »
    See 1 Publication for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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