Key features and details
- Rabbit polyclonal to DDX5 (phospho Y593)
- Suitable for: ELISA, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DDX5 (phospho Y593) antibody
See all DDX5 primary antibodies
DescriptionRabbit polyclonal to DDX5 (phospho Y593)
Specificityab62255 detects endogenous levels of DDX5 only when phosphorylated at tyrosine 593.
Tested applicationsSuitable for: ELISA, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic phosphopeptide derived from human DDX5 around the phosphorylation site of tyrosine 593 (Q-A-YP-A-Y).
- ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab62255 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab62255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/500 - 1/1000.|
FunctionRNA-dependent ATPase activity. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. May be involved in pre-mRNA splicing.
Sequence similaritiesBelongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
modificationsArg-502 is dimethylated, probably to asymmetric dimethylarginine.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- ATP dependent RNA helicase DDX5 antibody
- DDX 5 antibody
- Ddx5 antibody
ab62255 has been referenced in 1 publication.
- Arun G et al. mrhl RNA, a long noncoding RNA, negatively regulates Wnt signaling through its protein partner Ddx5/p68 in mouse spermatogonial cells. Mol Cell Biol 32:3140-52 (2012). PubMed: 22665494