Overview

  • Product name

    Anti-DDX50 antibody [EPR5272]
    See all DDX50 primary antibodies
  • Description

    Rabbit monoclonal [EPR5272] to DDX50
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human DDX50 aa 50-150. The exact sequence is proprietary.
    Database link: Q9BQ39

  • Positive control

    • HeLa, 293T, K562 and Jurkat whole cell lysate (ab7899)
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/200000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Sequence similarities

      Belongs to the DEAD box helicase family. DDX21/DDX50 subfamily.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • Cellular localization

      Nucleus > nucleolus.
    • Information by UniProt
    • Database links

    • Alternative names

      • 4933429B04Rik antibody
      • ATP-dependent RNA helicase DDX50 antibody
      • Ddx50 antibody
      • DDX50_HUMAN antibody
      • DEAD (Asp-Glu-Ala-Asp) box polypeptide 50 antibody
      • DEAD box protein 50 antibody
      • Gu beta antibody
      • Gu-beta antibody
      • GU2 antibody
      • GUB antibody
      • MGC109605 antibody
      • MGC3199 antibody
      • Nucleolar protein Gu2 antibody
      • RH II antibody
      • RH II/GuB antibody
      • RNA helicase II / Gu beta antibody
      see all

    Images

    • All lanes : Anti-DDX50 antibody [EPR5272] (ab133507) at 1/50000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 83 kDa
      Observed band size: 83 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab133507 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133507.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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