Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5273] to DDX50
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Product nameAnti-DDX50 antibody [EPR5273]
See all DDX50 primary antibodies
DescriptionRabbit monoclonal [EPR5273] to DDX50
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human DDX50 aa 600-700. The exact sequence is proprietary.
Database link: Q9BQ39
- HeLa, 293T, K562 and Jurkat whole cell lysate (ab7899); Human thyroid gland adenocarcinoma tissue
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109515 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Sequence similaritiesBelongs to the DEAD box helicase family. DDX21/DDX50 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- 4933429B04Rik antibody
- ATP-dependent RNA helicase DDX50 antibody
- Ddx50 antibody
All lanes : Anti-DDX50 antibody [EPR5273] (ab109515) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : K562 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 83 kDa
Observed band size: 83 kDa
Immunohistochemical analysis of DDX50 in paraffin embedded Human thyroid gland adenocarcinoma tissue, using ab109515 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ab109515 has not yet been referenced specifically in any publications.