Key features and details
- Rabbit polyclonal to DDX51
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DDX51 antibody
DescriptionRabbit polyclonal to DDX51
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla
Synthetic peptide cprresponding to a region between residues 616 and 666 of human DDX51 (NP_778236.1)
- HeLa whole cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab72292 was affinity purified using an epitope specific to DDX51 immobilized on solid support.
Our Abpromise guarantee covers the use of ab72292 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
RelevanceDDX51 belongs to the DEAD box helicase family. It is thought to be an ATP-binding RNA helicase involved in the biogenesis of 60S ribosomal subunits. Helicases are involved in unwinding nucleic acids. The DEAD box helicases are involved in various aspects of RNA metabolism, including nuclear transcription, pre mRNA splicing, ribosome biogenesis, nucleocytoplasmic transport, translation, RNA decay and organellar gene expression.
Cellular localizationNuclear, nucleolus.
- DDX 51 antibody
- DEAD (Asp Glu Ala Asp) box polypeptide 51 antibody
- DEAD box protein 51 antibody
All lanes : Anti-DDX51 antibody (ab72292) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 72 kDa
Observed band size: 72 kDa
Additional bands at: 172 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Detection of DDX51 by Western Blot of Immunprecipitate.
Lane 1: ab72292, at 1µg/ml, staining DDX51 in HeLa whole cell lysate immunoprecipitated using ab72292 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded).
Lane 2: Control IgG. Detection: Chemiluminescence with an exposure time of 3 minutes.
ab72292 has not yet been referenced specifically in any publications.