The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
Use at 2-5 µg/mg of lysate.
DDX51 belongs to the DEAD box helicase family. It is thought to be an ATP-binding RNA helicase involved in the biogenesis of 60S ribosomal subunits. Helicases are involved in unwinding nucleic acids. The DEAD box helicases are involved in various aspects of RNA metabolism, including nuclear transcription, pre mRNA splicing, ribosome biogenesis, nucleocytoplasmic transport, translation, RNA decay and organellar gene expression.
Detection of DDX51 by Western Blot of Immunprecipitate.
Lane 1: ab72292, at 1µg/ml, staining DDX51 in HeLa whole cell lysate immunoprecipitated using ab72292 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded).
Lane 2: Control IgG.
Detection: Chemiluminescence with an exposure time of 3 minutes.