Key features and details
- Rabbit polyclonal to DDX54
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DDX54 antibody
DescriptionRabbit polyclonal to DDX54
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residues 625 and 675 of human DDX54 (NP_076977.3)
- HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab76947 was affinity purified using an epitope specific to DDX54 immobilized on solid support.
Our Abpromise guarantee covers the use of ab76947 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 99 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionHas RNA-dependent ATPase activity. Represses the transcriptional activity of nuclear receptors.
Sequence similaritiesBelongs to the DEAD box helicase family. DDX54/DBP10 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- 2410015A15Rik antibody
- AI414901 antibody
- ATP dependent RNA helicase DDX54 antibody
All lanes : Anti-DDX54 antibody (ab76947) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 99 kDa
Observed band size: 99 kDa
Additional bands at: 180 kDa, 230 kDa, 72 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling DDX54 with ab76947 at 1/200 (1µg/ml). Detection: DAB.
ab76947 at 1µg/ml staining DDX54 in HeLa whole cell lysate immunoprecipitated using ab76947 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
ab76947 has not yet been referenced specifically in any publications.