Anti-DDX55 antibody (ab109591)
Key features and details
- Rabbit polyclonal to DDX55
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-DDX55 antibody -
Description
Rabbit polyclonal to DDX55 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide, corresponding to a region between residue 175 and 225 of Human DDX55 (NP_065987.1)
-
Positive control
- Whole cell lysate from HeLa or 293T cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109591 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/2000 - 1/10000. Predicted molecular weight: 69 kDa.
|
|
IP |
Use at 2-5 µg/mg of lysate.
|
Notes |
---|
WB
1/2000 - 1/10000. Predicted molecular weight: 69 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
-
Function
Probable ATP-binding RNA helicase. -
Sequence similarities
Belongs to the DEAD box helicase family. DDX55/SPB4 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Domain
The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis. - Information by UniProt
-
Database links
- Entrez Gene: 57696 Human
- SwissProt: Q8NHQ9 Human
- Unigene: 286173 Human
-
Alternative names
- ATP dependent RNA helicase DDX55 antibody
- ATP-dependent RNA helicase DDX55 antibody
- DDX 55 antibody
see all
Images
-
All lanes : Anti-DDX55 antibody (ab109591) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 69 kDa
Exposure time: 30 seconds -
Detection of DDX55 by Western Blot of Immunprecipitate.
ab109591 at 0.4µg/ml staining DDX55 in HeLa whole cell lysate immunoprecipitated using ab109591 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab109591 has not yet been referenced specifically in any publications.