Key features and details
- Rabbit polyclonal to DDX55
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DDX55 antibody
See all DDX55 primary antibodies
DescriptionRabbit polyclonal to DDX55
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region between residue 175 and 225 of Human DDX55 (NP_065987.1)
- Whole cell lysate from HeLa or 293T cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab109591 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 69 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionProbable ATP-binding RNA helicase.
Sequence similaritiesBelongs to the DEAD box helicase family. DDX55/SPB4 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
DomainThe Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.
- Information by UniProt
- ATP dependent RNA helicase DDX55 antibody
- ATP-dependent RNA helicase DDX55 antibody
- DDX 55 antibody
All lanes : Anti-DDX55 antibody (ab109591) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 69 kDa
Exposure time: 30 seconds
Detection of DDX55 by Western Blot of Immunprecipitate.
ab109591 at 0.4µg/ml staining DDX55 in HeLa whole cell lysate immunoprecipitated using ab109591 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
ab109591 has not yet been referenced specifically in any publications.