Key features and details
- Rabbit polyclonal to DDX55 - C-terminal
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DDX55 antibody - C-terminal
See all DDX55 primary antibodies
DescriptionRabbit polyclonal to DDX55 - C-terminal
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey
- WB: RT-4 whole cell lysates; IHC-P: Human testis tissue; ICC: U-2 OS whole cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab187895 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
FunctionProbable ATP-binding RNA helicase.
Sequence similaritiesBelongs to the DEAD box helicase family. DDX55/SPB4 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
DomainThe Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.
- Information by UniProt
- ATP dependent RNA helicase DDX55 antibody
- ATP-dependent RNA helicase DDX55 antibody
- DDX 55 antibody
Immunohistochemical analysis of paraffin-embedded human testis tissue labeling DDX55 in the nucleus and cytoplasm of seminiferus ducts subset cells with ab187895 at a 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemical analysis U-2 OS (Human bone osteosarcoma epithelial cell line) whole cells of labeling DDX55 in the nucleus and nucleoli with ab187895 at 2 µg/ml.
Anti-DDX55 antibody - C-terminal (ab187895) at 0.4 µg/ml + RT4 (Human urinary bladder cancer cell line) whole cell lysate
ab187895 has not yet been referenced specifically in any publications.