Product nameAnti-DDX56/DDX21 antibody
See all DDX56/DDX21 primary antibodies
DescriptionRabbit polyclonal to DDX56/DDX21
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Human DDX56/DDX21 aa 497-547.
Database link: Q9NY93
- HeLa and 293T cell lysates.
This product was previously labelled as DDX56
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab115158 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 62 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMay play a role in later stages of the processing of the pre-ribosomal particles leading to mature 60S ribosomal subunits. Has intrinsic ATPase activity.
Tissue specificityDetected in heart, brain, liver, pancreas, placenta and lung.
Sequence similaritiesBelongs to the DEAD box helicase family. DDX56/DBP9 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- 61 kd nucleolar helicase antibody
- ATP-dependent 61 kDa nucleolar RNA helicase antibody
- DDX21 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling DDX56/DDX21 with ab115158 at 1/1000 (1µg/ml). Detection: DAB.
Immunoprecipitation of DDX56/DDX21 (3 µg of antibody per mg of HeLa cell lysate; 20% of the IP loaded). Lane 1-Immunoprecipitation with an anti-DDX56/DDX21 antibody that recognizes an upstream epitope; Lane 2- Immunoprecipitation with anti-DDX56/DDX21 antibody (ab115158); Lane 3- Immunoprecipitation with IgG control. Anti-DDX56/DDX21 antibody (ab115158) used at 1 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 30 sec.
All lanes : Anti-DDX56/DDX21 antibody (ab115158) at 0.1 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Predicted band size: 62 kDa
Exposure time: 3 minutes
ab115158 has not yet been referenced specifically in any publications.