Overview

  • Product name

  • Description

    Rabbit polyclonal to DDX56/DDX21
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to Human DDX56/DDX21 aa 251-403. (AAH01235)

  • Positive control

    • A549, HeLa, HepG2 and HCT116 cell lines; MCF7 xenograft ICC/IF: HeLa cells.
  • General notes

     This product was previously labelled as DDX56

     

Properties

Applications

Our Abpromise guarantee covers the use of ab97648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.
WB 1/500 - 1/3000. Predicted molecular weight: 62 kDa.
ICC/IF 1/100 - 1/500.

Target

  • Function

    May play a role in later stages of the processing of the pre-ribosomal particles leading to mature 60S ribosomal subunits. Has intrinsic ATPase activity.
  • Tissue specificity

    Detected in heart, brain, liver, pancreas, placenta and lung.
  • Sequence similarities

    Belongs to the DEAD box helicase family. DDX56/DBP9 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus > nucleolus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 61 kd nucleolar helicase antibody
    • ATP-dependent 61 kDa nucleolar RNA helicase antibody
    • DDX21 antibody
    • DDX26 antibody
    • Ddx56 antibody
    • DDX56_HUMAN antibody
    • DEAD (Asp-Glu-Ala-Asp) box helicase 56 antibody
    • DEAD (Asp-Glu-Ala-Asp) box polypeptide 56 antibody
    • DEAD box protein 21 antibody
    • DEAD box protein 56 antibody
    • DEAD box RNA helicase antibody
    • DEAD/H box 56 antibody
    • NOH61 antibody
    • Nucleolar helicase of 61 kDa antibody
    • Probable ATP-dependent RNA helicase DDX56 antibody
    • Putative nucleolar RNA helicase antibody
    see all

Images

  • Paraformaldehyde-fixed HeLa cells stained for DDX56/DDX21 using ab97648 at 1/500 dilution in ICC/IF (green). Alpha tubulin filaments are detected with an ant-tubulin antibody (red).

  • Anti-DDX56/DDX21 antibody (ab97648) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 62 kDa



    7.5% SDS PAGE
  • ab97648, at a 1/200 dilution, staining DDX56/DDX21 in paraformaldehyde fixed HeLa cells by Immunofluorescence. The lower image is costained with Hoechst 33342.

  • ab97648, at a 1/500 dilution, staining DDX56/DDX21 in formalin fixed, paraffin embedded MCF7 xenograft by Immunohistochemistry.

References

ab97648 has not yet been referenced specifically in any publications.

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