Key features and details
- Rabbit polyclonal to DDX56/DDX21
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DDX56/DDX21 antibody
See all DDX56/DDX21 primary antibodies
DescriptionRabbit polyclonal to DDX56/DDX21
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Recombinant fragment corresponding to Human DDX56/DDX21 aa 251-403. (AAH01235)
- A549, HeLa, HepG2 and HCT116 cell lines; MCF7 xenograft ICC/IF: HeLa cells.
This product was previously labelled as DDX56
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97648 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500.|
|WB||1/500 - 1/3000. Predicted molecular weight: 62 kDa.|
|ICC/IF||1/100 - 1/500.|
FunctionMay play a role in later stages of the processing of the pre-ribosomal particles leading to mature 60S ribosomal subunits. Has intrinsic ATPase activity.
Tissue specificityDetected in heart, brain, liver, pancreas, placenta and lung.
Sequence similaritiesBelongs to the DEAD box helicase family. DDX56/DBP9 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- 61 kd nucleolar helicase antibody
- ATP-dependent 61 kDa nucleolar RNA helicase antibody
- DDX21 antibody
Paraformaldehyde-fixed HeLa cells stained for DDX56/DDX21 using ab97648 at 1/500 dilution in ICC/IF (green). Alpha tubulin filaments are detected with an ant-tubulin antibody (red).
Anti-DDX56/DDX21 antibody (ab97648) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 62 kDa
7.5% SDS PAGE
ab97648, at a 1/200 dilution, staining DDX56/DDX21 in paraformaldehyde fixed HeLa cells by Immunofluorescence. The lower image is costained with Hoechst 33342.
ab97648, at a 1/500 dilution, staining DDX56/DDX21 in formalin fixed, paraffin embedded MCF7 xenograft by Immunohistochemistry.
ab97648 has been referenced in 1 publication.
- Li D et al. DDX56 inhibits type I interferon by disrupting assembly of IRF3-IPO5 to inhibit IRF3 nucleus import. J Cell Sci 133:N/A (2019). PubMed: 31340999