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Rabbit polyclonal to DDX59
Predicted to work with:
Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide, corresponding to a region between residue 569 and 619 of Human DDX59 (NP_001026895.2)
HeLa or 293T whole cell lysate
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7-8
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 69 kDa.
Use at 2-5 µg/mg of lysate.
Belongs to the DEAD box helicase family. DDX59 subfamily.
Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Contains 1 HIT-type zinc finger.
The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.
Information by UniProt
DDX 59 antibody
Western blot - Anti-DDX59 antibody (ab109592)
All lanes : Anti-DDX59 antibody (ab109592) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 69 kDa Exposure time: 3 minutes
Immunoprecipitation - Anti-DDX59 antibody (ab109592)
Detection of DDX59 by Western Blot of Immunprecipitate.
ab109592 at 1µg/ml staining DDX59 in HeLa whole cell lysate immunoprecipitated using ab109592 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
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