Key features and details
- Rabbit polyclonal to DDX6
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-DDX6 antibody
See all DDX6 primary antibodies
DescriptionRabbit polyclonal to DDX6
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chicken, Cow, Xenopus laevis, Xenopus tropicalis
Recombinant fragment corresponding to Human DDX6 aa 1-236.
MSTARTENPV IMGLSSQNGQ LRGPVKPTGG PGGGGTQTQQ QMNQLKNTNT INNGTQQQAQ SMTTTIKPGD DWKKTLKLPP KDLRIKTSDV TSTKGNEFED YCLKRELLMG IFEMGWEKPS PIQEESIPIA LSGRDILARA KNGTGKSGAY LIPLLERLDL KKDNIQAMVI VPTRELALQV SQICIQVSKH MGGAKVMATT GGTNLRDDIM RLDDTVHVVI ATPGRILDLI KKGVAK
Database link: P26196
- WB: HeLa cells ICC/IF: HeLa cells IHC-P: Ca922 xenograft tissue. Mouse stomach.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 10% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab151984 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 54 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).|
|ICC/IF||1/100 - 1/1000.|
RelevanceDEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis and cellular growth and division. In the process of mRNA degradation, DDX6 may play a role in mRNA decapping. It forms a complex with DCP1A, DCP2, EDC3 and EDC4/HEDLS.
Cellular localizationCytoplasm; P-body. Note: Processing bodies (PB).
- ATP dependent RNA helicase DDX6 antibody
- ATP dependent RNA helicase p54 antibody
- DDX6 antibody
Paraffin embedded mouse stomach tissue stained for DDX6 using ab151984 at 1/500 dilution in immunohistochemical analysis.
HeLa cells stained for DDX6 (green) using ab151984 at 1/500 dilution in ICC/IF.
Anti-DDX6 antibody (ab151984) at 1/1000 dilution + Hela whole cell lysate
at 30 µg
Predicted band size: 54 kDa
10% SDS PAGE
Immunohistochemical analysis of paraffin embedded Ca922 (Human gingival carcinoma) xenograft tissue labeling DDX6 with ab151984 at 1/100.
ab151984 has not yet been referenced specifically in any publications.