Overview

  • Product name

  • Description

    Goat polyclonal to DDX6
  • Host species

    Goat
  • Tested applications

    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity

    Reacts with: Human, Pig
    Predicted to work with: Mouse, Guinea pig, Dog
  • Immunogen

    Synthetic peptide corresponding to Human DDX6 aa 471-483 (C terminal).
    Sequence:

    C-AEYHSEPVEDEKP


    Database link: P26196

  • Positive control

    • Jurkat cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab45869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration.

Antibody detection limit dilution 1:128,000.

WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).

Target

  • Relevance

    DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis and cellular growth and division. In the process of mRNA degradation, DDX6 may play a role in mRNA decapping. It forms a complex with DCP1A, DCP2, EDC3 and EDC4/HEDLS.
  • Cellular localization

    Cytoplasm; P-body. Note: Processing bodies (PB).
  • Database links

  • Alternative names

    • ATP dependent RNA helicase DDX6 antibody
    • ATP dependent RNA helicase p54 antibody
    • DDX6 antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 6 antibody
    • DEAD box 6 antibody
    • DEAD box protein 6 antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 6 (RNA helicase 54kD) antibody
    • FLJ36338 antibody
    • HLR2 antibody
    • Oncogene RCK antibody
    • P54 antibody
    • Probable ATP-dependent RNA helicase DDX6 antibody
    • RCK antibody
    see all

Images

  • All lanes : Anti-DDX6 antibody (ab45869) at 0.3 µg/ml

    Lane 1 : Human Burkitt's lymphoma cell line
    Lane 2 : Human T cell leukemia cells from peripheral blood
    Lane 3 : Human liver hepatocellular carcinoma

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 54 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human cortex staining DDX6 with ab45869at 5 μg/ml

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human thymus staining DDX6 with ab45869at 5 μg/ml

  • Anti-DDX6 antibody (ab45869) at 0.3 µg/ml + Jurkat cell lysate (35µg protein in RIPA buffer).

    Predicted band size: 54 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour. Detected by chemiluminescence.

References

This product has been referenced in:

  • Rajgor D  et al. NMDAR-dependent Argonaute 2 phosphorylation regulates miRNA activity and dendritic spine plasticity. EMBO J 37:N/A (2018). Read more (PubMed: 29712715) »
  • Shen X  et al. Identification of novel SHPS-1-associated proteins and their roles in regulation of insulin-like growth factor-dependent responses in vascular smooth muscle cells. Mol Cell Proteomics : (2009). WB ; Pig . Read more (PubMed: 19299420) »
See all 2 Publications for this product

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