Key features and details
- Rabbit polyclonal to DDX6
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DDX6 antibody
See all DDX6 primary antibodies
DescriptionRabbit polyclonal to DDX6
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a sequence between N terminal residues 1 to 50 of human DDX6 (Swiss-Prot P26196).
- Whole cell lysate from HeLa cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab70455 was affinity purified using an epitope specific to DDX6 immobilized on solid support.
Our Abpromise guarantee covers the use of ab70455 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/5000 - 1/15000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
RelevanceDEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis and cellular growth and division. In the process of mRNA degradation, DDX6 may play a role in mRNA decapping. It forms a complex with DCP1A, DCP2, EDC3 and EDC4/HEDLS.
Cellular localizationCytoplasm; P-body. Note: Processing bodies (PB).
- ATP dependent RNA helicase DDX6 antibody
- ATP dependent RNA helicase p54 antibody
- DDX6 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling DDX6 with ab70455 at 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-DDX6 antibody (ab70455) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Predicted band size: 54 kDa
Observed band size: 54 kDa
250µg HeLa whole cell lysate treated with ab70455 at 0.04 µg/ml for the Western blot, and at 3 µg/mg lysate for Immunoprecipitation.
ab70455 has been referenced in 1 publication.
- Booy EP et al. Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR. Nucleic Acids Res 46:11575-11591 (2018). PubMed: 30247708