Overview

  • Product name

  • Description

    Rabbit polyclonal to DDX6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    A synthetic peptide corresponding to a region between residues 1 and 50 of human DDX6 (SwissProt entry P26196; GeneID 1656).

  • Positive control

    • Human: breast carcinoma, colon carcinoma, non-small cell lung cancer, ovarian carcinoma, prostate carcinoma, stomach adenocarcinoma and testicular seminoma Mouse: colon carcinoma CT26, renal cell carcinoma and teratoma

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab87321 is affinity purified using an epitope specific to DDX6 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab87321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with tris EDTA pH 9.0 buffer will enhance staining.
    Likely to work with frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis and cellular growth and division. In the process of mRNA degradation, DDX6 may play a role in mRNA decapping. It forms a complex with DCP1A, DCP2, EDC3 and EDC4/HEDLS.
    • Cellular localization

      Cytoplasm; P-body. Note: Processing bodies (PB).
    • Database links

    • Alternative names

      • ATP dependent RNA helicase DDX6 antibody
      • ATP dependent RNA helicase p54 antibody
      • DDX6 antibody
      • DEAD (Asp Glu Ala Asp) box polypeptide 6 antibody
      • DEAD box 6 antibody
      • DEAD box protein 6 antibody
      • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 6 (RNA helicase 54kD) antibody
      • FLJ36338 antibody
      • HLR2 antibody
      • Oncogene RCK antibody
      • P54 antibody
      • Probable ATP-dependent RNA helicase DDX6 antibody
      • RCK antibody
      see all

    Images

    • ab87321, at a 1/250 dilution, staining DDX6 in formalin fixed, paraffin embedded human prostate carcinoma tissue by Immunohistochemistry. Detection: by DAB staining.
    • ab87321, at a 1/250 dilution, staining DDX6 in formalin fixed, paraffin embedded mouse renal cell carcinoma tissue by Immunohistochemistry. Detection: by DAB staining.

    References

    ab87321 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab87321.
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