Overview

  • Product name

    Anti-Deadpan antibody [11D1BC7]
    See all Deadpan primary antibodies
  • Description

    Rat monoclonal [11D1BC7] to Deadpan
  • Host species

    Rat
  • Tested applications

    Suitable for: ICC/IF, IHCmore details
  • Species reactivity

    Reacts with: Drosophila melanogaster
  • Immunogen

    Recombinant full length protein. This information is considered to be commercially sensitive.

  • Positive control

    • Drosophila larval brain, Drosophila stage 11 embryos.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: HEPES buffered saline
  • Concentration information loading...
  • Purification notes

    The purity of ab195173 is near homogeneity, as judged by SDS-PAGE. ab195173 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. >95% based on SDS-PAGE.
  • Clonality

    Monoclonal
  • Clone number

    11D1BC7
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab195173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
IHC Use at an assay dependent concentration.

Target

  • Relevance

    Transcriptional repressor of genes that require a bHLH protein for their transcription. Likely to be involved in the functional rather than the morphological differentiation of neurons. Loss of DPN function results in weak motor activity, lethargic behavior, and death. Implicated in sex determination as Groucho-DPN complex act directly to repress SXL transcription
  • Cellular localization

    Nuclear
  • Database links

    • Alternative names

      • deadpan antibody
      • Dpn antibody
      • Protein deadpan antibody

    Images

    • Immunohistochemistry analysis using ab195173 detecting the nuclear transcription factor Deadpan protein in the neuroblasts of the third instar larval brain of Drosophila melanogaster. The brain was fixed in 4% formaldehyde (20 min) and then incubated in 1%BSA / 10% normal goat serum in 0.1% PBS-Tween for 1hr to permeabilise the cells and block non-specific protein-protein interactions. The brain was then incubated with ab195173 (10 µg/mL) overnight at +4°C and detected with Alexa Fluor® 555 goat anti-rat IgG. The counterstain was chicken anti-GFP detected with Alexa Fluor® 488 used according to manufacturer’s instructions.

    • Immunohistochemistry analysis using ab195173 detecting Drosophila stage 11 embryos stained showing Deadpan (Dpn) protein in all neuroblast nuclei. The Deadpan stain used ab195173 at 10 µg/mL and detected with Alexa Fluor® 405 conjugated goat anti-rat (at 10µg/mL).

    References

    This product has been referenced in:

    • Sato K  et al. Partial proteasomal degradation of Lola triggers the male-to-female switch of a dimorphic courtship circuit. Nat Commun 10:166 (2019). Read more (PubMed: 30635583) »
    • Mirkovic M  et al. Induced aneuploidy in neural stem cells triggers a delayed stress response and impairs adult life span in flies. PLoS Biol 17:e3000016 (2019). Read more (PubMed: 30794535) »
    See all 8 Publications for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Sample
    Fruit fly (Drosophila melanogaster) Cell (Wing Imaginal disc)
    Specification
    Wing Imaginal disc
    Permeabilization
    Yes - 0.5% Triton-X100
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Mar 16 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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